caa a22 4500 467895236 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9020-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9020-3 Evolutionary divergence of LFY function in the mustards Arabidopsis thaliana and Leavenworthia crassa [Elektronische Daten] [M. Sliwinski, M. White, A. Maizel, D. Weigel, D. Baum] LEAFY (LFY), a transcription factor involved in the regulation of flower development in Arabidopsis thaliana, has been identified as a candidate gene in the diversification of plant architecture in Brassicaceae. Previous research with Leavenworthia crassa, which produces solitary flowers in the axils of rosette leaves, has shown that the L. crassa LFY ortholog, LcrLFY, rescues most aspects of flower development in A. thaliana but showed two novel traits: flowers produced additional petals and inflorescences produced terminal flowers. In this paper, we explore the molecular mechanisms responsible for these novel phenotypes. We used microarray hybridizations to identify 32 genes differentially expressed between a transgenic LcrLFY line and a control transgenic LFY line. Of particular interest, TERMINAL FLOWER 1 (TFL1) transcripts were found at elevated levels in LcrLFY lines. To distinguish regulatory versus functional changes within the LcrLFY locus, reciprocal chimeric transgenes between LcrLFY and LFY were constructed. These lines implicate divergence of LcrLFY cis-regulation as the primary cause of both novel transgenic phenotypes but implicate divergence of LcrLFY protein function as the primary cause of elevated TFL1 levels. Taken together these results show that LcrLFY has diverged from A.␣thaliana in both the cis-regulatory and protein-coding regions and imply that molecular coevolution of LcrLFY and the L. crassa TFL1 ortholog, LcrTFL1, contributed to the evolution of rosette flowering. Springer Science+Business Media B.V., 2006 Leavenworthia crassa nationallicence LEAFY nationallicence TERMINAL FLOWER 1 nationallicence Evolutionary developmental biology nationallicence Inflorescence nationallicence Rosette flowering nationallicence Sliwinski M. Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, WI, USA aut White M. Laboratory of Genetics, University of Wisconsin, 425-G Henry Mall, 53706, Madison, WI, USA aut Maizel A. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen, Germany aut Weigel D. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen, Germany aut Baum D. Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, WI, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 279-289 0167-4412 62:1-2<279 2006 62 11103 https://doi.org/10.1007/s11103-006-9020-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9020-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sliwinski M. Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- White M. Laboratory of Genetics, University of Wisconsin, 425-G Henry Mall, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Maizel A. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen, Germany aut NATIONALLICENCE 700 1- Weigel D. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen, Germany aut NATIONALLICENCE 700 1- Baum D. Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, WI, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 279-289 0167-4412 62:1-2<279 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer