caa a22 4500 467895260 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9014-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9014-1 A novel cis -acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter [Elektronische Daten] [Claudia Vickers, Gangping Xue, Peter Gresshoff] To examine the genetic controls of endosperm (ES) specificity, several cereal seed storage protein (SSP) promoters were isolated and studied using a transient expression analysis system. An oat globulin promoter (AsGlo1) capable of driving strong ES-specific expression in barley and wheat was identified. Progressive 5′ deletions and cis element mutations demonstrated that the mechanism of specificity in the AsGlo1 promoter was distinct from that observed in glutelin and prolamin promoters. A novel interrupted palindromic sequence, ACATGTCATCATGT, was required for ES specificity and substantially contributed to expression strength of the␣AsGlo1 promoter. This sequence was termed the endosperm specificity palindrome (ESP) element. The GCN4 element, which has previously been shown to be required for ES specificity in cereal SSP promoters, had a quantitative role but was not required for tissue specificity. The 960-bp AsGlo1 promoter and a 251-bp deletion containing the ESP element also drove ES-specific expression in stably transformed barley. Reporter gene protein accumulated at very high levels (10% of total soluble protein) in ES tissues of plants transformed with an AsGlo1:GFP construct. Expression strength and tissue specificity were maintained over five transgenic generations. These attributes make the AsGlo1 promoter an ideal promoter for biotechnology applications. In conjunction with previous findings, our data demonstrate that there is more than one genetically distinct mechanism by which ES specificity can be achieved in cereal SSP promoters, and also suggest that there is redundancy between transcriptional and post-transcriptional tissue specificity mechanisms in cereal globulin genes. Springer Science+Business Media B.V., 2006 AsGlo1 promoter nationallicence ESP element nationallicence Endosperm specificity nationallicence GCN4 box nationallicence Globulin promoter nationallicence Promoter analysis nationallicence ABA : abscisic acid nationallicence Act1 : rice actin gene nationallicence AsGlo1 : oat 12S globulin gene nationallicence bZIP : basic leucine zipper protein nationallicence DAP : days after pollination nationallicence ES : endosperm nationallicence ESP : endosperm specificity palindrome nationallicence GA : gibberellin nationallicence GFP : green fluorescent protein nationallicence GluB-1 : rice B1 glutelin gene nationallicence Glu-B1 : wheat high-molecular weight glutenin gene nationallicence GP : Hordeum vulgare cv. Golden Promise nationallicence GUS Plus : modified Staphylococcus sp. β-glucuronidase nationallicence Hor2-4 : barley B1 hordein gene nationallicence Hor3−1 : barley D hordein gene nationallicence SSP : seed storage protein nationallicence sUTR : synthetic untranslated leader nationallicence TC : tissue culture nationallicence TEP : total extractable protein nationallicence TSP : total soluble protein nationallicence XYN : xylanase nationallicence Vickers Claudia CSIRO Plant Industry, 306 Carmody Rd, 4067, St Lucia, Brisbane, Australia aut Xue Gangping CSIRO Plant Industry, 306 Carmody Rd, 4067, St Lucia, Brisbane, Australia aut Gresshoff Peter ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, 4072, St Lucia, Australia aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 195-214 0167-4412 62:1-2<195 2006 62 11103 https://doi.org/10.1007/s11103-006-9014-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9014-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vickers Claudia CSIRO Plant Industry, 306 Carmody Rd, 4067, St Lucia, Brisbane, Australia aut NATIONALLICENCE 700 1- Xue Gangping CSIRO Plant Industry, 306 Carmody Rd, 4067, St Lucia, Brisbane, Australia aut NATIONALLICENCE 700 1- Gresshoff Peter ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, 4072, St Lucia, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 195-214 0167-4412 62:1-2<195 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer