caa a22 4500 467895279 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9001-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9001-6 Loss of NECROTIC SPOTTED LESIONS 1 associates with cell death and defense responses in Arabidopsis thaliana [Elektronische Daten] [Yoshiteru Noutoshi, Takashi Kuromori, Takuji Wada, Takashi Hirayama, Asako Kamiya, Yuko Imura, Michiko Yasuda, Hideo Nakashita, Ken Shirasu, Kazuo Shinozaki] We isolated a lesion mimic mutant, n ecrotic s potted l esions 1 (nsl1), from Ds-tagged Arabidopsis thaliana accession No-0. The nsl1 mutant exhibits a growth retardation phenotype and develops spotted necrotic lesions on its rosette and cauline leaves. These phenotypes occur in the absence of pathogens indicating that nsl1 mutants may constitutively express defense responses. Consistent with this idea, nsl1 accumulates high levels of callose and autofluorescent phenolic compounds localized to the necrotic lesions. Furthermore RNA gel blot analysis revealed that genes associated with disease resistance activation are upregulated in the nsl1 mutants and these plants contain elevated levels of salicylic acid (SA). Crossing nsl1 with an SA deficient mutant, eds16-1, revealed that the nsl1 lesions and growth retardation are dependent upon SA. The nsl1 phenotypes are not suppressed under either the rar1-10 or sgt1b-1 genetic background. NSL1 encodes a novel 612aa protein which contains a membrane-attack complex/perforin (MACPF) domain, which is conserved in bacteria, fungi, mammals and plants. The possible modes of action of NSL1 protein in negative regulation of cell death programs and defense responses are discussed. Springer Science+Business Media B.V., 2006 Cell death nationallicence Defense responses nationallicence Salicylic acid nationallicence MACPF domain nationallicence Avr : avirulence nationallicence CNGC : cyclic nucleotide-gated cation channel nationallicence Ds : Dissociation transposon nationallicence H2O2 : hydrogen peroxide nationallicence HR : hypersensitive response nationallicence LRR : leucine rich repeat nationallicence MACPF : membrane attack complex and perforin nationallicence NahG : salicylate hydroxylase nationallicence MAP kinase : mitogen-activated protein kinase nationallicence NBS : nucleotide binding site nationallicence PCD : programmed cell death nationallicence PCR : polymerase chain reaction nationallicence PR genes : pathogenesis-related genes nationallicence R gene : disease resistance gene nationallicence ROS : reactive oxygen species nationallicence SA : salicylic acid nationallicence SAR : systemic acquired resistance nationallicence TIR : Toll/interleukin-1 receptor nationallicence Noutoshi Yoshiteru Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai Tsukuba, 305-0074, Ibaraki, Japan aut Kuromori Takashi Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Wada Takuji Plant Science Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Hirayama Takashi Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Kamiya Asako Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Imura Yuko Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Yasuda Michiko Plant Functions Laboratory, RIKEN Institute, Wako-shi, 351-0198, Saitama, Japan aut Nakashita Hideo Plant Functions Laboratory, RIKEN Institute, Wako-shi, 351-0198, Saitama, Japan aut Shirasu Ken Plant Science Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut Shinozaki Kazuo Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai Tsukuba, 305-0074, Ibaraki, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 29-42 0167-4412 62:1-2<29 2006 62 11103 https://doi.org/10.1007/s11103-006-9001-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9001-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Noutoshi Yoshiteru Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai Tsukuba, 305-0074, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kuromori Takashi Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Wada Takuji Plant Science Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Hirayama Takashi Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Kamiya Asako Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Imura Yuko Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Yasuda Michiko Plant Functions Laboratory, RIKEN Institute, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Nakashita Hideo Plant Functions Laboratory, RIKEN Institute, Wako-shi, 351-0198, Saitama, Japan aut NATIONALLICENCE 700 1- Shirasu Ken Plant Science Center, RIKEN Yokohama Institute, Suehiro-cho 1-7-22, Tsurumi-ku, 230-0045, Yokohama, Japan aut NATIONALLICENCE 700 1- Shinozaki Kazuo Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai Tsukuba, 305-0074, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 29-42 0167-4412 62:1-2<29 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer