caa a22 4500 467895325 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9006-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9006-1 ASK1 , a SKP1 homolog, is required for nuclear reorganization, presynaptic homolog juxtaposition and the proper distribution of cohesin during meiosis in Arabidopsis [Elektronische Daten] [Dazhong Zhao, Xiaohui Yang, Li Quan, Ljudmilla Timofejeva, Nathan Rigel, Hong Ma, Christopher Makaroff] Nuclear reorganization and juxtaposition of homologous chromosomes at late leptotene/early zygotene are essential steps before chromosome synapsis at pachytene. We report the results of detailed studies, which demonstrate that nuclear reorganization and homolog juxtapositioning processes are defective in a null mutant, ask1-1. Our results from 4, 6-diamino-2-phenylindole (DAPI)-stained spreads showed that the "synizetic knot”, which is typically found in wild type (WT) meiosis during late leptotene and zygotene, was missing in the ask1-1 mutant. Furthermore, ask1-1 meiocytes exhibited only limited homolog juxtaposition at centromere regions at early zygotene. Immunodetection of the cohesin protein SYN1 identified ask1 defects in cohesin distribution from zygotene to anaphase I. Analysis of meiotic chromosomes in ask1-1 and syn1 single mutants, as well as an ask1-1 syn1 double mutant indicate that ASK1 is required for normal SYN1 distribution during meiotic prophase I and suggest that ask1 associated defects may be primarily related to SYN1 mislocalization. Springer Science+Business Media B.V., 2006 Arabidopsis thaliana nationallicence ASK1 nationallicence Homolog juxtaposition nationallicence Meiosis nationallicence SYN1 synizetic knot nationallicence Cohesin nationallicence Synapsis nationallicence AE : axial element nationallicence CE : central element nationallicence DAPI : 4, 6-diamino-2-phenylindole nationallicence LEs : lateral elements nationallicence SC : synaptonemal complex nationallicence TEM : transmission electron microscopy nationallicence Zhao Dazhong Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Yang Xiaohui Department of Chemistry and Biochemistry, Miami University, Hughes Hall, 45056, Oxford, Ohio, USA aut Quan Li Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Timofejeva Ljudmilla Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Rigel Nathan Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Ma Hong Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Makaroff Christopher Department of Chemistry and Biochemistry, Miami University, Hughes Hall, 45056, Oxford, Ohio, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 99-110 0167-4412 62:1-2<99 2006 62 11103 https://doi.org/10.1007/s11103-006-9006-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9006-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Dazhong Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Yang Xiaohui Department of Chemistry and Biochemistry, Miami University, Hughes Hall, 45056, Oxford, Ohio, USA aut NATIONALLICENCE 700 1- Quan Li Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Timofejeva Ljudmilla Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Rigel Nathan Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Ma Hong Department of Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Makaroff Christopher Department of Chemistry and Biochemistry, Miami University, Hughes Hall, 45056, Oxford, Ohio, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 99-110 0167-4412 62:1-2<99 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer