caa a22 4500 467895333 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9011-4 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9011-4 Subcellular pyrophosphate metabolism in developing tubers of potato ( Solanum tuberosum ) [Elektronische Daten] [Eva Farré, Susanne Tech, Richard Trethewey, Alisdair Fernie, Lothar Willmitzer] PPi has previously been implicated specifically in the co-ordination of the sucrose-starch transition and in the broader context of its role as co-factor in heterotrophic plant metabolism. In order to assess the compartmentation of pyrophosphate (PPi) metabolism in the potato tuber we analysed the effect of expressing a bacterial pyrophosphatase in the amyloplast of wild type tubers or in the cytosol or amyloplast of invertase-expressing tubers. The second and third approaches were adopted since we have previously characterized the invertase expressing lines to both exhibit highly altered sucrose metabolism and to contain elevated levels of PPi (Farré etal. (2000a) Plant Physiol 123:681) and therefore this background rendered questions concerning the level of communication between the plastidic and cytosolic pyrophosphate pools relatively facile. In this study we observed that the increase in PPi in the invertase expressing lines was mainly confined to the cytosol. Accordingly, the expression of a bacterial pyrophosphatase in the plastid of either wild type or invertase-expressing tubers did not lead to a decrease in total PPi content. However, the expression of the heterologous pyrophosphatase in␣the cytosol of cytosolic invertase-expressing tubers led to strong metabolic changes. These results are discussed both with respect to our previous hypotheses and to current models of the compartmentation of potato tuber metabolism. Springer Science+Business Media B.V., 2006 Pyrophosphate nationallicence Pyrophosphatase nationallicence Potato ( Solanum tuberosum ) nationallicence Tuber metabolism nationallicence Metabolic compartmentation nationallicence Carbon metabolism nationallicence Farré Eva Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut Tech Susanne Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut Trethewey Richard Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut Fernie Alisdair Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut Willmitzer Lothar Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 165-179 0167-4412 62:1-2<165 2006 62 11103 https://doi.org/10.1007/s11103-006-9011-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9011-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Farré Eva Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut NATIONALLICENCE 700 1- Tech Susanne Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut NATIONALLICENCE 700 1- Trethewey Richard Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut NATIONALLICENCE 700 1- Fernie Alisdair Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut NATIONALLICENCE 700 1- Willmitzer Lothar Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 165-179 0167-4412 62:1-2<165 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer