caa a22 4500 467895341 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9013-2 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9013-2 Gene Expression Patterns During Somatic Embryo Development and Germination in Maize Hi II Callus Cultures [Elektronische Daten] [Ping Che, Tanzy Love, Bronwyn Frame, Kan Wang, Alicia Carriquiry, Stephen Howell] Gene expression patterns were profiled during somatic embryogenesis in a regeneration-proficient maize hybrid line, Hi II, in an effort to identify genes that might be used as developmental markers or targets to optimize regeneration steps for recovering maize plants from tissue culture. Gene expression profiles were generated from embryogenic calli induced to undergo embryo maturation and germination. Over 1,000 genes in the 12,060 element arrays showed significant time variation during somatic embryo development. A substantial number of genes were downregulated during embryo maturation, largely histone and ribosomal protein genes, which may result from a slowdown in cell proliferation and growth during embryo maturation. The expression of these genes dramatically recovered at germination. Other genes up-regulated during embryo maturation included genes encoding hydrolytic enzymes (nucleases, glucosidases and proteases) and a few storage genes (an α-zein and caleosin), which are good candidates for developmental marker genes. Germination is accompanied by the up-regulation of a number of stress response and membrane transporter genes, and, as expected, greening is associated with the up-regulation of many genes encoding photosynthetic and chloroplast components. Thus, some, but not all genes typically associated with zygotic embryogenesis are significantly up or down-regulated during somatic embryogenesis in Hi II maize line regeneration. Although many genes varied in expression throughout somatic embryo development in this study, no statistically significant gene expression changes were detected between total embryogenic callus and callus enriched for transition stage somatic embryos. Springer Science+Business Media B.V., 2006 Somatic embryogenesis nationallicence Microarray nationallicence Transcriptome nationallicence Plant regeneration nationallicence Tissue culture nationallicence Corn nationallicence Che Ping Plant Sciences Institute, Iowa State University, 50011, Ames, IA, USA aut Love Tanzy Department of Statistics, Iowa State University, 50011, Ames, IA, USA aut Frame Bronwyn Center for Plant Transformation and the Department of Agronomy, Iowa State University, 50011, Ames, IA, USA aut Wang Kan Center for Plant Transformation and the Department of Agronomy, Iowa State University, 50011, Ames, IA, USA aut Carriquiry Alicia Department of Statistics, Iowa State University, 50011, Ames, IA, USA aut Howell Stephen Plant Sciences Institute, Iowa State University, 50011, Ames, IA, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 1-14 0167-4412 62:1-2<1 2006 62 11103 https://doi.org/10.1007/s11103-006-9013-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9013-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Che Ping Plant Sciences Institute, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Love Tanzy Department of Statistics, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Frame Bronwyn Center for Plant Transformation and the Department of Agronomy, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Wang Kan Center for Plant Transformation and the Department of Agronomy, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Carriquiry Alicia Department of Statistics, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Howell Stephen Plant Sciences Institute, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 1-14 0167-4412 62:1-2<1 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer