caa a22 4500 46789535X CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9022-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9022-1 Localization and Quantification of Plasma Membrane Aquaporin Expression in Maize Primary Root: A Clue to Understanding their Role as Cellular Plumbers [Elektronische Daten] [Charles Hachez, Menachem Moshelion, Enric Zelazny, Damien Cavez, François Chaumont] Water movement across root tissues occurs by parallel apoplastic, symplastic, and transcellular pathways that the plant can control to a certain extent. Because water channels or aquaporins (AQPs) play an important role in regulating water flow, studies on AQP mRNA and protein expression in different root tissues are essential. Here, we quantified and localized the expression of Zea mays plasma membrane AQPs (ZmPIPs) in primary root tip using in situ and quantitative RT-PCR and immunodetection approaches. All ZmPIP genes except ZmPIP2;7 were expressed in primary roots. Expression was found to be dependent on the developmental stage of the root, with, in general, an increase in expression towards the elongation and mature zones. Two genes, ZmPIP1;5 and ZmPIP2;5, showed the greatest increase in expression (up to 11- and 17-fold, respectively) in the mature zone, where they accounted for 50% of the total expressed ZmPIPs. The immunocytochemical localization of ZmPIP2;1 and ZmPIP2;5 in the exodermis and endodermis indicated that they are involved in root radial water movement. In addition, we detected a polar localization of ZmPIP2;5 to the external periclinal side of epidermal cells in root apices, suggesting an important role in water uptake from the root surface. Finally, protoplast swelling assays showed that root cells display a variable, but globally low, osmotic water permeability coefficient (P f<10µm/s). However, the presence of a population of cells with a higher P f (up to 26µm/s) in mature zone of the root might be correlated with the increased expression of several ZmPIP genes. Springer Science+Business Media B.V., 2006 Aquaporin nationallicence PIP nationallicence mRNA and protein expression nationallicence Root water movement nationallicence ABA : abscisic acid nationallicence AQP : aquaporin nationallicence Lpr : root hydraulic conductivity nationallicence P f : osmotic water permeability coefficient nationallicence RT-PCR : reverse transcription-PCR nationallicence ZmPIP : Zea mays plasma membrane intrinsic protein nationallicence Hachez Charles Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut Moshelion Menachem Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut Zelazny Enric Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut Cavez Damien Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut Chaumont François Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 305-323 0167-4412 62:1-2<305 2006 62 11103 https://doi.org/10.1007/s11103-006-9022-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9022-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hachez Charles Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Moshelion Menachem Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Zelazny Enric Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Cavez Damien Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Chaumont François Unité de Biochimie physiologique, Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 5-15, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 305-323 0167-4412 62:1-2<305 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer