caa a22 4500 467895376 CHVBK 20180406152802.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9008-z doi (NATIONALLICENCE)springer-10.1007/s11103-006-9008-z Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing [Elektronische Daten] [Chengwei Li, Yuling Bai, Evert Jacobsen, Richard Visser, Pim Lindhout, Guusje Bonnema] Oidium neolycopersici is a causal agent of tomato powdery mildew. In this paper, gene expression profiles were investigated of susceptible, monogenic- and polygenic resistant tomato genotypes in response to O. neolycopersici infection by using cDNA-AFLP. Around 30,000 TDFs (Transcript Derived Fragments), representing ∼22% of the transcriptome based on in silico estimation, were identified and 887 TDFs were differentially expressed (DE-TDFs) upon inoculation with O. neolycopersici spores. Forty-two percent of the identified DE-TDFs were detected in both the compatible and incompatible interactions, a subset of these were studied for their temporal patterns. All of these common induced DE-TDFs displayed an expression peak at 7days post incoluation in monogenic resistant response but sustained up-regulation in the susceptible and the polygenic resistant response. While more than half of these common DE-TDFs showed earlier timing in incompatible interactions compared to compatible interaction. Only 2% of the identified DE-TDFs were specific to either the monogenic or the polygenic resistant response. By annotation of the 230 sequenced DE-TDFs we found that 34% of the corresponding transcripts were known to be involved in plant defense, whereas the other transcripts played general roles in signal transduction (11%), regulation (24%), protein synthesis and degradation (11%), energy metabolism (12%) including photosynthesis, photorespiration and respiration. Springer Science+Business Media B.V., 2006 Basal defense nationallicence cDNA-AFLP nationallicence Monogenic resistance nationallicence Oidium neolycopersici nationallicence Polygenic resistance nationallicence Solanum lycopersicum nationallicence DE-TDF : Differentially expressed TDF nationallicence DPI : Days post inoculation nationallicence HPI : Hours post inoculation nationallicence TDF : Transcript derived fragment nationallicence HR : Hypersensitive response nationallicence Li Chengwei Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Bai Yuling Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Jacobsen Evert Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Visser Richard Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Lindhout Pim Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Bonnema Guusje Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 127-140 0167-4412 62:1-2<127 2006 62 11103 https://doi.org/10.1007/s11103-006-9008-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9008-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Chengwei Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bai Yuling Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Jacobsen Evert Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Visser Richard Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Lindhout Pim Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bonnema Guusje Laboratory of Plant Breeding, Wageningen University, Postbus 386, 6700 AJ, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 127-140 0167-4412 62:1-2<127 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer