caa a22 4500 467895392 CHVBK 20180406152802.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9007-0 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9007-0 The C-terminal region of α′ subunit of soybean β-conglycinin contains two types of vacuolar sorting determinants [Elektronische Daten] [Keito Nishizawa, Nobuyuki Maruyama, Shigeru Utsumi] In maturing seed cells, proteins that accumulate in the protein storage vacuoles (PSVs) are synthesized on the endoplasmic reticulum (ER) and transported by vesicles to the PSVs. Vacuolar sorting determinants (VSDs) which are usually amino acid sequences of short or moderate length direct the proteins to this pathway. VSDs identified so far are classified into two types: sequence specific VSDs (ssVSDs) and C-terminal VSDs (ctVSDs). We previously demonstrated that VSDs of α′ and β subunits of β-conglycinin, one of major storage proteins of soybean (Glycine max), reside in the C-terminal ten amino acids. Here we show that both types of VSDs coexist within this region of the α′ subunit. Although ctVSDs can function only at the very C-termini of proteins, the C-terminal ten amino acids of α′ subunit directed green fluorescent protein (GFP) to the PSVs even when they were placed at the N-terminus of GFP, indicating that an ssVSD resides in the sequence. By mutation analysis, it was found that the core sequence of the ssVSD is Ser-Ile-Leu (fifth to seventh residues counted from the C-terminus) which is conserved in the α and β subunits and some vicilin-like proteins. On the other hand, the sequence composed of the C-terminal three amino acids (AFY) directed GFP to the PSVs when it was placed at the C-terminus of GFP, though the function as a VSD was disrupted at the N-terminus of GFP, indicating that the AFY sequence is a ctVSD. Springer Science+Business Media B.V. 2006, 2006 β-Conglycinin nationallicence Protein sorting nationallicence Protein storage vacuole nationallicence Secretory pathway nationallicence Soybean nationallicence Vacuolar sorting determinant nationallicence Nishizawa Keito Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Maruyama Nobuyuki Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Utsumi Shigeru Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 111-125 0167-4412 62:1-2<111 2006 62 11103 https://doi.org/10.1007/s11103-006-9007-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9007-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishizawa Keito Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Maruyama Nobuyuki Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Utsumi Shigeru Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 111-125 0167-4412 62:1-2<111 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer