caa a22 4500 467895414 CHVBK 20180406152802.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9002-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9002-5 Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco [Elektronische Daten] [Jan Lochman, Vladimir Mikes] Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine-ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. Collected data shows that ergosterol is able to activate the expression of a number of defence genes and could increase resistance against pathogens. Springer Science+Business Media B.V., 2006 Defence genes nationallicence Nicotiana tabacum nationallicence Ergosterol nationallicence Elicitor nationallicence Salicylic acid nationallicence Plant defence nationallicence Lochman Jan Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic aut Mikes Vladimir Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 43-51 0167-4412 62:1-2<43 2006 62 11103 https://doi.org/10.1007/s11103-006-9002-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9002-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lochman Jan Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic aut NATIONALLICENCE 700 1- Mikes Vladimir Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 43-51 0167-4412 62:1-2<43 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer