caa a22 4500 467899819 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0032-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0032-2 AFLP analysis of nephthytis ( Syngonium podophyllum Schott) selected from somaclonal variants [Elektronische Daten] [J. Chen, R. Henny, P. Devanand, C. Chao] This study analyzed genetic differences of 19 cultivars selected from somaclonal variants of Syngonium podophyllum Schott along with their parents as well as seven additional Syngonium species and six other aroids using amplified fragment length polymorphism (AFLP) markers generated by 12 primer sets. Among the 19 somaclonal cultivars, ‘Pink Allusion' was selected from ‘White Butterfly'. Tissue culture of ‘Pink Allusion' through organogenesis resulted in the development of 13 additional cultivars. Self-pollination of ‘Pink Allusion' obtained a cultivar, ‘Regina Red Allusion', and tissue culture propagation of ‘Regina Red Allusion' led to the release of five other cultivars. The 12 primer sets generated a total of 1,583 scorable fragments from all accessions, of which 1,284 were polymorphic (81.9%). The percentages of polymorphic fragments within ‘White Butterfly' and ‘Regina Red Allusion' groups, however, were only 1.2% and 0.4%, respectively. Jaccard's similarity coefficients among somaclonal cultivars derived from ‘White Butterfly' and ‘Regina Red Allusion', on average, were 0.98 and 0.99, respectively. Seven out of the 15 cultivars from the ‘White Butterfly' group and three out of six from the ‘Regina Red Allusion' group were clearly distinguished by AFLP analysis as unique fragments were associated with respective cultivars. The unsuccessful attempt to distinguish the remaining eight cultivars from the ‘White Butterfly' group and three from the ‘Regina Red Allusion' group was not attributed to experimental errors or the number of primer sets used; rather it is hypothesized to be caused by DNA methylation and/or some rare mutations. This study also calls for increased genetic diversity of cultivated Syngonium as they are largely derived from somaclonal variants. Springer-Verlag, 2005 AFLP analysis nationallicence Aroids nationallicence Ornamental foliage plants nationallicence Somaclonal variants nationallicence Syngonium nationallicence AFLP: Amplified fragment length polymorphism nationallicence PCOA: Principal coordinate analysis nationallicence PCR: Polymerase chain reaction nationallicence UPGMA: Unweighted pair group method with arithmetic averages nationallicence Chen J. Environmental Horticulture Department and Mid-Florida Research and Education Center, University of Florida, IFAS, 2725 Binion Road, 32703-8504, Apopka, FL, USA aut Henny R. Environmental Horticulture Department and Mid-Florida Research and Education Center, University of Florida, IFAS, 2725 Binion Road, 32703-8504, Apopka, FL, USA aut Devanand P. Department of Botany and Plant Sciences, University of California - Riverside, 92521-0124, Riverside, CA, USA aut Chao C. Department of Botany and Plant Sciences, University of California - Riverside, 92521-0124, Riverside, CA, USA aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 743-749 0721-7714 24:12<743 2006 24 299 https://doi.org/10.1007/s00299-005-0032-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0032-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen J. Environmental Horticulture Department and Mid-Florida Research and Education Center, University of Florida, IFAS, 2725 Binion Road, 32703-8504, Apopka, FL, USA aut NATIONALLICENCE 700 1- Henny R. Environmental Horticulture Department and Mid-Florida Research and Education Center, University of Florida, IFAS, 2725 Binion Road, 32703-8504, Apopka, FL, USA aut NATIONALLICENCE 700 1- Devanand P. Department of Botany and Plant Sciences, University of California - Riverside, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Chao C. Department of Botany and Plant Sciences, University of California - Riverside, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 743-749 0721-7714 24:12<743 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer