caa a22 4500 467899827 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0049-6 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0049-6 A comparison between hairy root cultures and wild plants of Saussurea involucrata in phenylpropanoids production [Elektronische Daten] [Chun-Xiang Fu, Yan-jun Xu, De-Xiu Zhao, Feng Ma] Saussurea involucrata is an important medicinal plant that produces a few bioactive secondary metabolites, such as hispidulin, rutin, and syringin. Previously, we established a hairy root culture system for this species through Agrobacterium-mediated transformation. The present study addressed the issue as how hairy root cultures perform in phenylpronoid accumulation. From the ethanolic extract of a hairy root culture established for Saussurea involucrata, syringin, rutin and hispidulin, were isolated and their chemical structures were confirmed by HPLC-ESI-MS. A quantitative study of the compounds showed great levels of syringin and hispidulin (being 43.5±1.13 and 0.34±0.023mgg−1 dry weight, respectively), about 40 and 3 times, respectively, higher than those from wild plants. But, the levels of rutin from hairy roots were much lower (0.71±0.043 vs. 6.59±0.56mgg−1 dry weight). Compared with untransformed root cultures, syringin and hispidulin levels were also higher. An experiment on culture media showed that MS was superior to others for phenylpropanoids accumulation in hairy roots, a 28-day culture produced 405mgl−1 syringin. Springer-Verlag, 2005 Hairy root nationallicence Hispidulin nationallicence Rutin nationallicence Saussurea involucrata nationallicence Syringin nationallicence BA: 6-benzyladenine nationallicence DAD: photodiode array detection nationallicence HPLC-ESI-MS: high performance liquid chromatography-electrospray ionization-mass spectrometry nationallicence HPLC: high performance liquid chromatography nationallicence IAA: indole-3-acetic acid nationallicence LOD: limit of detection nationallicence Fu Chun-Xiang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093,, Xiangshan, Beijing, China aut Xu Yan-jun College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, 100094,, Haidian, Beijing, China aut Zhao De-Xiu Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093,, Xiangshan, Beijing, China aut Ma Feng Department of Biology, University of Waterloo, N2L 3G1,, Waterloo, Ontario, Canada aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 750-754 0721-7714 24:12<750 2006 24 299 https://doi.org/10.1007/s00299-005-0049-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0049-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fu Chun-Xiang Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093,, Xiangshan, Beijing, China aut NATIONALLICENCE 700 1- Xu Yan-jun College of Science, China Agricultural University, 2 Yuanmingyuan Xi Lu, 100094,, Haidian, Beijing, China aut NATIONALLICENCE 700 1- Zhao De-Xiu Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093,, Xiangshan, Beijing, China aut NATIONALLICENCE 700 1- Ma Feng Department of Biology, University of Waterloo, N2L 3G1,, Waterloo, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 750-754 0721-7714 24:12<750 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer