caa a22 4500 467899835 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0016-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0016-2 High-frequency plant regeneration through cyclic secondary somatic embryogenesis in black pepper ( Piper nigrum L.) [Elektronische Daten] [R. Nair, S. Dutta Gupta] A high-frequency plantlet regeneration protocol was developed for black pepper (Piper nigrum L.) through cyclic secondary somatic embryogenesis. Secondary embryos formed from the radicular end of the primary somatic embryos which were originally derived from micropylar tissues of germinating seeds on growth regulator-free SH medium in the absence of light. The process of secondary embryogenesis continued in a cyclic manner from the root pole of newly formed embryos resulting in clumps of somatic embryos. Strength of the medium and sucrose concentration influenced the process of secondary embryogenesis and fresh weight of somatic embryo clumps. Full-strength SH medium supplemented with 1.5% sucrose produced significantly higher fresh weight and numbers of secondary somatic embryos while 3.0 and 4.5% sucrose in the medium favored further development of proliferated embryos into plantlets. Ontogeny of secondary embryos was established by histological analysis. Secondary embryogenic potential was influenced by the developmental stage of the explanted somatic embryo and stages up to "torpedo” were more suitable. A single-flask system was standardized for proliferation, maturation, germination and conversion of secondary somatic embryos in suspension cultures. The system of cyclic secondary somatic embryogenesis in black pepper described here represents a permanent source of embryogenic material that can be used for genetic manipulations of this crop species. Springer-Verlag, 2005 Direct somatic embryogenesis nationallicence Piperaceae nationallicence Recurrent embryogenesis nationallicence Spice nationallicence Suspension culture nationallicence PGR : Plant growth regulator nationallicence PE : Primary somatic embryo nationallicence SE : Secondary somatic embryo nationallicence SH : Schenk and Hildebrandt (1972) nationallicence Nair R. Indian Institute of Spices Research, 673012, Calicut, Kerala, India aut Dutta Gupta S. Agricultural and Food Engineering Department, Indian Institute of Technology, 721302, Kharagpur, West Bengal, India aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 699-707 0721-7714 24:12<699 2006 24 299 https://doi.org/10.1007/s00299-005-0016-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0016-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nair R. Indian Institute of Spices Research, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Dutta Gupta S. Agricultural and Food Engineering Department, Indian Institute of Technology, 721302, Kharagpur, West Bengal, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 699-707 0721-7714 24:12<699 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer