caa a22 4500 467899843 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0013-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0013-5 Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat ( Triticum aestivum L.) microspore culture [Elektronische Daten] [Jocelyne Letarte, Ecaterina Simion, Mai Miner, Ken Kasha] The objective of this study was to improve induction of embryogenesis in wheat microspore culture in order to obtain a high number of regenerable embryos. The arabinogalactan (AG) Larcoll and the arabinogalactan-protein (AGP) from gum arabic were tested on two spring genotypes to see if they could increase microspore viability and induce embryogenesis in the microspore culture. Adding Larcoll significantly decreased microspore mortality in both genotypes regardless of the presence or absence of ovaries in the culture. Similarly, gum arabic had a strong effect on the number of embryos produced and regenerated green plants. In fact, by using only gum arabic we were able to obtain green plants from wheat microspore cultures without the presence of ovaries. In addition to preventing a high mortality rate of the cells, our results show that the induction of embryogenesis in wheat microspore cultures is strongly affected by the use of both AG or AGP. Springer-Verlag, 2005 Gum arabic nationallicence Haploid nationallicence Larcoll nationallicence Polysaccharide nationallicence Programmed cell death nationallicence Wheat doubled haploids nationallicence AGs : Arabinogalactan nationallicence AGPs : Arabinogalactan-protein nationallicence DHs : Doubled haploid nationallicence ELSs : Embryo-like-structures nationallicence PCDs : Programmed cell death nationallicence Letarte Jocelyne Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut Simion Ecaterina Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut Miner Mai Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut Kasha Ken Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 691-698 0721-7714 24:12<691 2006 24 299 https://doi.org/10.1007/s00299-005-0013-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0013-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Letarte Jocelyne Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut NATIONALLICENCE 700 1- Simion Ecaterina Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut NATIONALLICENCE 700 1- Miner Mai Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut NATIONALLICENCE 700 1- Kasha Ken Department of Plant Agriculture, University of Guelph, N1G 2W1, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 691-698 0721-7714 24:12<691 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467899843 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer