caa a22 4500 467899851 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0023-3 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0023-3 Efficient Agrobacterium tumefaciens -mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants [Elektronische Daten] [Géraldine Blanc, Christelle Baptiste, Gérald Oliver, Florence Martin, Pascal Montoro] An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA105 harbouring the vector pCAMBIA2301. Transformation capacity and competence of the embryogenic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20°C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryogenesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged. Springer-Verlag, 2005 Agrobacterium tumefaciens nationallicence Cocultivation nationallicence Cryopreservation nationallicence Genetic transformation nationallicence GUS nationallicence Rubber tree nationallicence Temperature nationallicence 3,4-D : 3,4-Dichlorophenoxyacetic acid nationallicence ABA : Abscisic acid nationallicence BAP : Benzylaminopurine nationallicence DEV : Development medium nationallicence EXP : Expression medium nationallicence FM : Fresh matter nationallicence GR : Growth regulator nationallicence GUS : β-Glucuronidase nationallicence MM : Maintenance medium nationallicence Blanc Géraldine Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut Baptiste Christelle Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut Oliver Gérald Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut Martin Florence Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut Montoro Pascal Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 724-733 0721-7714 24:12<724 2006 24 299 https://doi.org/10.1007/s00299-005-0023-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0023-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blanc Géraldine Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut NATIONALLICENCE 700 1- Baptiste Christelle Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut NATIONALLICENCE 700 1- Oliver Gérald Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut NATIONALLICENCE 700 1- Martin Florence Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut NATIONALLICENCE 700 1- Montoro Pascal Tree Crops Department, CIRAD, TA 80/03, Avenue d'Agropolis, 34 398, Montpellier, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 724-733 0721-7714 24:12<724 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer