caa a22 4500 46789986X CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0019-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0019-z Stable transformation of mature zygotic embryos and regeneration of transgenic plants of chir pine ( Pinus roxbughii Sarg.) [Elektronische Daten] [V. Parasharami, V. Naik, S. von Arnold, R. Nadgauda, D. Clapham] A particle inflow gun was used to transfer the plasmid pAHC25 containing the bar gene conferring resistance to glufosinate and the gusA reporter gene, each driven by the maize ubiquitin promoter, to mature embryos of Pinus roxburghii (chir pine). High levels of transient expression were obtained when embryos were cultured for 6 days on 10μM benzyl adenine-containing medium and then exposed to high osmoticum (0.5M sucrose) before and after bombardment. Selection on medium containing Basta enabled recovery of stably transformed shoots, both from the epicotyl and from adventitious buds. The primary transformed shoots from the epicotyl were multiplied via axillary shoots. Transformation was confirmed by histochemical staining for β-glucuronidase (GUS) activity, by polymerase chain reaction (PCR) amplification of fragments of gusA and nos terminator, and by the resistance of needles to Basta. Springer-Verlag, 2005 Conifer nationallicence Glufosinate nationallicence Particle bombardment nationallicence Pinus roxburghii nationallicence Stable transformation nationallicence GUS : β-glucuronidase nationallicence X-Gluc : 5-bromo-4-choloro-3-indolyl-β-d-glucuronic acid nationallicence BA : benzylaminopurine nationallicence Parasharami V. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut Naik V. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut von Arnold S. Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden aut Nadgauda R. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut Clapham D. Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 708-714 0721-7714 24:12<708 2006 24 299 https://doi.org/10.1007/s00299-005-0019-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0019-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parasharami V. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Naik V. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- von Arnold S. Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Nadgauda R. Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Clapham D. Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 708-714 0721-7714 24:12<708 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer