caa a22 4500 467899878 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00299-005-0030-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0030-4 Cloning and characterization of a novel splicing isoform of the iron-superoxide dismutase gene in rice ( Oryza sativa L.) [Elektronische Daten] [Wang Feng, Wang Hongbin, Liu Bing, Wang Jinfa] Superoxide dismutases (SODs) are ubiquitous metalloenzymes in aerobic organisms that play a crucial role in protecting organisms against ROS. Here, we report the molecular cloning and functional characterization of a novel alternatively spliced variant of the iron-superoxide dismutase gene, OsFe-SODb, from a rice panicle cDNA library. The alternative splicing event occurred in the fourth exon of the OsFe-SOD gene, and led to the translation of two isoforms of different sizes. The 5′ flanking region of the OsFe-SOD was cloned and many cis-acting regulatory elements were found that are involved in light responsiveness, including a G-box and an I-box. RT-PCR analysis showed that the two alternative forms of OsFe-SOD were expressed in both the vegetative and reproductive tissues of Cpslo17. Moreover, accumulation of both isoforms was upregulated by light induction. In addition, the alternative splicing of OsFe-SOD mRNA was sensitive to low temperature. High yield production of the two recombinant OsFe-SOD isoforms was achieved in Escherichia coli. SOD assays showed that C-terminal truncation in OsFe-SODb did not result in a loss of SOD enzyme activity. Springer-Verlag, 2005 Rice ( Oryza sativa L.) nationallicence Iron-superoxide dismutase nationallicence cDNA nationallicence Alternative splicing nationallicence RT-PCR nationallicence SOD assay nationallicence SOD: Superoxide dismutase nationallicence ROS: Reactive oxygen species nationallicence RT-PCR: Reverse transcription-polymerase chain reaction nationallicence ORF: Open reading frame nationallicence bp: Base pair nationallicence kb: Kilobase pair nationallicence EST: Expressed sequence tag nationallicence Feng Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Hongbin Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Bing Liu The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Jinfa Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 734-742 0721-7714 24:12<734 2006 24 299 https://doi.org/10.1007/s00299-005-0030-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0030-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hongbin Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Bing Liu The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jinfa Wang The State Key Laboratory for Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 24/12(2006-01-01), 734-742 0721-7714 24:12<734 2006 24 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer