caa a22 4500 467899894 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0027-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0027-z Organogenesis and plant regeneration in Taxus wallichiana (Zucc.) [Elektronische Daten] [Mukul Datta, Anrini Majumder, Sumita Jha] We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength Lloyd and McCown's basal medium supplemented with SH vitamin (½ WPMSH), 0.5mgl−1 6-benzyladenine (BA) in combination with 1.0-2.0mgl−1 2,4-dichlorophenoxyacetic acid (2,4-D) or α-Napthaleneacetic acid (NAA) produced two morphologically distinct types of calli-compact, green callus (CG) and compact, yellow (CY) callus after 4 weeks of culture. Optimum frequency (63%) of adventitious shoot bud induction was achieved in CG callus (3.0±0.67 shoot buds per gram of CG callus) when cultured on ½ WPMSH basal medium supplemented with 2.5mgl−1 BA after 4 weeks. The inclusion of 1% activated charcoal (AC) to ½ WPMSH basal medium (shoot elongation medium) led to maximum shoot elongation (2.15cms). Microshoots rooted in high frequency (40%) in MS basal medium in which the concentration of nitrates was reduced to one-fifth the normal concentration after 4 months of culture. Springer-Verlag, 2005 Taxus wallichiana nationallicence Zygotic embryos nationallicence Organogenesis nationallicence Paclitaxel nationallicence BA : 6-Benzyladenine nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence NAA : α-Napthaleneacetic acid nationallicence AC : Activated charcoal nationallicence CG : Compact, green callus nationallicence CY : Compact, yellow callus nationallicence FC : Friable, creamish callus nationallicence IBA : Indole-3-butyric acid nationallicence IAA : Indole-3-acetic acid nationallicence Kn : Kinetin nationallicence 2iP : 2-Isopentyladenine nationallicence TDZ : Thidiazuron nationallicence Datta Mukul Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut Majumder Anrini Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut Jha Sumita Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 11-18 0721-7714 25:1<11 2006 25 299 https://doi.org/10.1007/s00299-005-0027-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0027-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Datta Mukul Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut NATIONALLICENCE 700 1- Majumder Anrini Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut NATIONALLICENCE 700 1- Jha Sumita Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 11-18 0721-7714 25:1<11 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer