caa a22 4500 467899924 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0024-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0024-2 Low light and low ammonium are key factors for guayule leaf tissue shoot organogenesis and transformation [Elektronische Daten] [Niu Dong, Belen Montanez, Robert Creelman, Katrina Cornish] A new method has been developed for guayule tissue culture and transformation. Guayule leaf explants have a poor survival rate when placed on normal MS medium and under normal culture room light conditions. Low light and low ammonium treatment greatly improved shoot organogenesis and transformation from leaf tissues. Using this method, a 35S promoter driven BAR gene and an ubiquitin-3 promoter driven GUS gene (with intron) have been successfully introduced into guayule. These transgenic guayule plants were resistant to the herbicide ammonium-glufosinate and were positive to GUS staining. Molecular analysis showed the expected band and signal in all GUS positive transformants. The transformation efficiency with glufosinate selection ranged from 3 to 6%. Transformation with a pBIN19-based plasmid containing a NPTII gene and then selection with kanamycin also works well using this method. The ratio of kanamycin-resistant calli to total starting explants reached 50% in some experiments. Springer-Verlag, 2005 Guayule nationallicence Parthenium argentatum nationallicence Agrobacterium nationallicence Transformation nationallicence Low light intensity nationallicence Low ammonium nationallicence Glufosinate nationallicence BAR nationallicence GUS nationallicence Dong Niu USDA/ARS/WRRC/CIU, 800 Buchanan St., 94710, Albany, CA, USA aut Montanez Belen Mendel Biotechnology Inc., 21375 Cabot Blvd., 94545, Hayward, CA, USA aut Creelman Robert Mendel Biotechnology Inc., 21375 Cabot Blvd., 94545, Hayward, CA, USA aut Cornish Katrina USDA/ARS/WRRC/CIU, 800 Buchanan St., 94710, Albany, CA, USA aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 26-34 0721-7714 25:1<26 2006 25 299 https://doi.org/10.1007/s00299-005-0024-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0024-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Niu USDA/ARS/WRRC/CIU, 800 Buchanan St., 94710, Albany, CA, USA aut NATIONALLICENCE 700 1- Montanez Belen Mendel Biotechnology Inc., 21375 Cabot Blvd., 94545, Hayward, CA, USA aut NATIONALLICENCE 700 1- Creelman Robert Mendel Biotechnology Inc., 21375 Cabot Blvd., 94545, Hayward, CA, USA aut NATIONALLICENCE 700 1- Cornish Katrina USDA/ARS/WRRC/CIU, 800 Buchanan St., 94710, Albany, CA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 26-34 0721-7714 25:1<26 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer