caa a22 4500 467899932 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0005-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0005-5 Generation of large numbers of transgenic Kentucky bluegrass ( Poa pratensis L.) plants following biolistic gene transfer [Elektronische Daten] [Caixia Gao, Li Jiang, Marianne Folling, Liebao Han, Klaus Nielsen] A very efficient transformation system, using biolistic bombardment, has been developed for the production of transgenic plants of Kentucky bluegrass (Poa pratensis L.). Embryogenic calli, initiated from immature embryos, were transformed either with pAct1IHPT-4 containing the hygromycin phosphotransferase (hpt) gene or with pDM803 containing the phosphinothricin acetyltransferase (bar) gene and the β-glucuronidase (uidA) gene. In total 119 independent transgenic plants were recovered from 153 hygromycin-resistant lines. Bialaphos selection yielded a total of 99 bialaphos-resistant lines and from these 34 independent transgenic plants were recovered. Southern blot analysis demonstrated the independent nature of the transgenic plants and also revealed a complex transgene integration pattern with multiple insertions. Springer-Verlag, 2005 Poa pratensis L nationallicence Grass nationallicence Biolistic bombardment nationallicence Embryogenic callus nationallicence Tissue culture nationallicence BAP : 6-Benzylaminopurine nationallicence 2,4-D : 2,4-Dichlorophenoxy acetic acid nationallicence uidA : ß-Glucuronidase nationallicence Gao Caixia DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut Jiang Li DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut Folling Marianne DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut Han Liebao Institute of Turfgrass Science, Beijing Forestry University, 10083, Beijing, P.R. China aut Nielsen Klaus DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 19-25 0721-7714 25:1<19 2006 25 299 https://doi.org/10.1007/s00299-005-0005-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0005-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Caixia DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut NATIONALLICENCE 700 1- Jiang Li DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut NATIONALLICENCE 700 1- Folling Marianne DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut NATIONALLICENCE 700 1- Han Liebao Institute of Turfgrass Science, Beijing Forestry University, 10083, Beijing, P.R. China aut NATIONALLICENCE 700 1- Nielsen Klaus DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660, Store Heddinge, Denmark aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 19-25 0721-7714 25:1<19 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer