caa a22 4500 467899940 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0031-3 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0031-3 GISH analysis of disomic Brassica napus - Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines [Elektronische Daten] [Youping Wang, Karin Sonntag, Eicke Rudloff, Peter Wehling, Rod Snowdon] Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding. Springer-Verlag, 2005 Brassica napus nationallicence Crambe abyssinica nationallicence Addition lines nationallicence Microspore culture nationallicence GISH nationallicence Wang Youping College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut Sonntag Karin Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut Rudloff Eicke Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut Wehling Peter Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut Snowdon Rod Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 35-40 0721-7714 25:1<35 2006 25 299 https://doi.org/10.1007/s00299-005-0031-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0031-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Youping College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Sonntag Karin Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 700 1- Rudloff Eicke Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 700 1- Wehling Peter Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany aut NATIONALLICENCE 700 1- Snowdon Rod Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 35-40 0721-7714 25:1<35 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer