caa a22 4500 467899959 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0034-0 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0034-0 Translation initiation by non-AUG codons in Arabidopsis thaliana transgenic plants [Elektronische Daten] [Annie Depeiges, Fabienne Degroote, Marie Espagnol, Georges Picard] The efficiency of translation initiation at codons differing at one or two nucleotides from AUG was tested as initiation codons for the phosphinotricin-acetyltransferase gene in T-DNA plant transformation in Arabidopsis thaliana. With the exception of UUA codon that differs from AUG at two nucleotides and does not permit any detectable activity, all the other codons (AUC, GUG, ACG, and CUG) present a phosphinotrycin acetyltransferase activity that varies between 5 and 10% of the AUG activity. This low activity is sufficient to confer glufosinate resistance to some of the plants. These results indicate that, in plants as is the case in animals, non-AUG initiating codons may be used for translation initiation, namely when a low expression rate is needed. Springer-Verlag, 2005 Plant nationallicence Arabidopsis thaliana nationallicence Non-AUG initiation nationallicence PAT gene nationallicence Depeiges Annie UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut Degroote Fabienne UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut Espagnol Marie UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut Picard Georges UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 55-61 0721-7714 25:1<55 2006 25 299 https://doi.org/10.1007/s00299-005-0034-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0034-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Depeiges Annie UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut NATIONALLICENCE 700 1- Degroote Fabienne UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut NATIONALLICENCE 700 1- Espagnol Marie UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut NATIONALLICENCE 700 1- Picard Georges UMR 6547 CNRS GEEM-BIOMOVE, Université Blaise Pascal, 24 avenue des Landais, 63177, Aubière, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 55-61 0721-7714 25:1<55 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer