caa a22 4500 467899975 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00299-005-0028-y doi (NATIONALLICENCE)springer-10.1007/s00299-005-0028-y Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper ( Capsicum annuum L.) [Elektronische Daten] [E. Supena, S. Suharsono, E. Jacobsen, J. Custers] Various systems of anther and microspore cultures were studied to establish an efficient doubled haploid production method for Indonesian hot pepper (Capsicum annuum L.). A shed-microspore culture protocol was developed which outperformed all the previously reported methods of haploid production in pepper. The critical factors of the protocol are: selection of flower buds with more than 50% late unicellular microspores, a 1 day 4°C pretreatment of the buds, followed by culture of the anthers in double-layer medium system for 1 week at 9°C and thereafter at 28°C in continuous darkness. The medium contained Nitsch components and 2% maltose, with 1% activated charcoal in the solid under layer and 2.5μM zeatin and 5μM indole-3-acetic acid in the liquid upper layer. All the ten genotypes of hot pepper tested, responded to this protocol. The best genotypes produced four to seven plants per original flower bud. This protocol can be used as a potential tool for producing doubled haploid plants for hot pepper breeding. Springer-Verlag, 2005 Capsicum annuum nationallicence Hot pepper nationallicence Shed-microspore culture nationallicence Defective shoots nationallicence Supena E. Research Center for Biotechnology, Bogor Agricultural University (IPB), P.O. Box 1, 16610, Bogor, Indonesia aut Suharsono S. Research Center for Biotechnology, Bogor Agricultural University (IPB), P.O. Box 1, 16610, Bogor, Indonesia aut Jacobsen E. Plant Research International, Wageningen University and Research Centre, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut Custers J. Plant Research International, Wageningen University and Research Centre, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 1-10 0721-7714 25:1<1 2006 25 299 https://doi.org/10.1007/s00299-005-0028-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0028-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Supena E. Research Center for Biotechnology, Bogor Agricultural University (IPB), P.O. Box 1, 16610, Bogor, Indonesia aut NATIONALLICENCE 700 1- Suharsono S. Research Center for Biotechnology, Bogor Agricultural University (IPB), P.O. Box 1, 16610, Bogor, Indonesia aut NATIONALLICENCE 700 1- Jacobsen E. Plant Research International, Wageningen University and Research Centre, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Custers J. Plant Research International, Wageningen University and Research Centre, P.O. Box 16, NL-6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/1(2006-02-01), 1-10 0721-7714 25:1<1 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer