caa a22 4500 467899983 CHVBK 20180406152814.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0091-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0091-4 Bacillus thuringiensis protein production, signal transduction, and insect control in chemically inducible PR-1a/cry1Ab broccoli plants [Elektronische Daten] [Jun Cao, Sarah Bates, Jian-Zhou Zhao, Anthony Shelton, Elizabeth Earle] In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cassette. Spraying leaves of these plants with the inducer acibenzolar-S-methyl (= 1,2,3 benzothiadiazole-7-thiocarboxylic acid-S-methyl-ester) (ASM) triggered expression of the cry1Ab gene and produced a high level of Cry1Ab protein within 2-3 days. Cry1Ab protein persisted in leaves for at least 8 weeks, providing prolonged protection from P. xylostella attack. Signals generated in inducer-treated leaves were transferred to untreated newly emerged leaves or heads, as seen by production of Cry1Ab protein and/or protection from insect damage in these plant parts. Signal transduction proceeded in an attenuated manner up to the sixth newly emerged leaf. No Cry1Ab protein was detectable by ELISA in uninduced young leaves, but small amounts of the protein were present in uninduced leaves older than 3 weeks and caused some insect mortality. Such basal expression of Bt genes without induction may favor the evolution of resistant insect populations and therefore limits the application of the PR-1a/cry1Ab system for insect management. However, the rapid production and steady maintenance of a high level of transgenic protein upon induction, the signal transduction observed, and the fact that the chemical inducer can be used in field conditions make the PR-1a promoter attractive for chemical regulation of other agriculturally or pharmaceutically important genes for which low expression in the absence of induction is not a concern. Springer-Verlag, 2005 Bacillus thuringiensis nationallicence Brassica oleracea nationallicence Chemically inducible nationallicence Cry1Ab protein nationallicence Diamondback moth nationallicence Plutella xylostella Linnaeus nationallicence PR-1a promoter nationallicence Signal transduction nationallicence Cao Jun Department of Plant Breeding and Genetics, Cornell University, 14853, Ithaca, NY, USA aut Bates Sarah Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut Zhao Jian-Zhou Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut Shelton Anthony Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut Earle Elizabeth Department of Plant Breeding and Genetics, Cornell University, 14853, Ithaca, NY, USA aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 554-560 0721-7714 25:6<554 2006 25 299 https://doi.org/10.1007/s00299-005-0091-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0091-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao Jun Department of Plant Breeding and Genetics, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Bates Sarah Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut NATIONALLICENCE 700 1- Zhao Jian-Zhou Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut NATIONALLICENCE 700 1- Shelton Anthony Department of Entomology, Cornell University/NYSAES, 14456, Geneva, NY, USA aut NATIONALLICENCE 700 1- Earle Elizabeth Department of Plant Breeding and Genetics, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 554-560 0721-7714 25:6<554 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer