caa a22 4500 467900000 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-006-0121-x doi (NATIONALLICENCE)springer-10.1007/s00299-006-0121-x Swapped green algal promoters: aphVIII -based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa [Elektronische Daten] [A. Hallmann, S. Wodniok] Production of transgenic organisms is a well-established, versatile course of action in molecular biology. Genetic engineering often requires heterologous, dominant antibiotic resistance genes that have been used as selectable markers in many species. However, as heterologous 5′ and 3′ flanking sequences often result in very low expression rates, endogenous flanking sequences, especially promoters, are mostly required and are easily obtained in model organisms, but it is much more complicated and time-consuming to get appropriate sequences from less common organisms. In this paper, we show that aminoglycoside 3′-phosphotransferase gene (aphVIII) based constructs with 3′ and 5′ untranslated flanking sequences (including promoters) from the multicellular green alga Volvox work in the unicellular green alga Chlamydomonas and flanking sequences from Chlamydomonas work in Volvox, at least if a low expression rate is compensated by an enforced high gene dosage. This strategy might be useful for all investigators that intend to transform species in which genomic sequences are not available, but sequences from related organisms exist. Springer-Verlag, 2006 Green algae nationallicence Volvocales nationallicence Recombinant DNA technology nationallicence Transgenic expression nationallicence Genetic transformation nationallicence Paromomycin resistance nationallicence aphVIII : Aminoglycoside 3′-phosphotran sferase (VIII) gene nationallicence hsp70A : Heat shock protein 70A ge ne nationallicence rbcS2 : Ribulose-1,5-bisphosphat-carboxylase small subunit gene 2 from Chlamydomonas reinhardtii nationallicence rbcS3 : Ribulose-1,5-bisphosphat-carboxylase small subunit gene 3 from Volvox carteri nationallicence UTR : Untranslated region nationallicence Hallmann A. Department of Cellular and Developmental Biology of Plants, University of Bielefeld, Universitätsstr 25, 33615, Bielefeld, Germany aut Wodniok S. Department of Cellular and Developmental Biology of Plants, University of Bielefeld, Universitätsstr 25, 33615, Bielefeld, Germany aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 582-591 0721-7714 25:6<582 2006 25 299 https://doi.org/10.1007/s00299-006-0121-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0121-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hallmann A. Department of Cellular and Developmental Biology of Plants, University of Bielefeld, Universitätsstr 25, 33615, Bielefeld, Germany aut NATIONALLICENCE 700 1- Wodniok S. Department of Cellular and Developmental Biology of Plants, University of Bielefeld, Universitätsstr 25, 33615, Bielefeld, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 582-591 0721-7714 25:6<582 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer