caa a22 4500 467900027 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0103-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0103-4 Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker—an endangered medicinal herb [Elektronische Daten] [S. Lattoo, S. Bamotra, R. Sapru Dhar, S. Khan, A. Dhar] An efficient in vitro multiplication system via multiple shoot bud induction and regeneration has been developed in Chlorophytum arundinaceum using shoot crown explants. Optimum regeneration frequency (87%) and desirable organogenetic response in the form of de novo organized multiple shoot buds without an intervening callus phase was obtained on Murashige and Skoog's (MS) minimal organics medium containing 3% sucrose (w/v) supplemented with 4×10−6M Kn and 2×10−6MIBA. Axenic secondary explants with multiple shoot buds on subculturing elicited best response with 1×10−5M Kinetin (Kn) and 5×10−6M indole-3-butyric acid (IBA) giving rise to an average of 18.74 shoots per culture with mean shoot length of 7.6cm ± 1.73. Varying molar ratios of either Kn/IBA or Kn/NAA revealed statistically significant differences in the regeneration frequencies among the phytohormone treatments. It was observed that the shoot bud differentiation and regeneration was influenced by the molar ratios of cytokinins/auxin rather than their relative concentrations. Healthy regenerated shoots were rooted in half strength MS basal medium containing 3% sucrose (w/v) supplemented with 5×10−6M IBA. Following simple hardening procedures, rooted plantlets, were transferred to soil-sand (1:1; v/v) with more than 90% success. Genetic fidelity was assessed using random amplified polymorphic DNA (RAPD), karyotype analysis and meiotic behaviour of in vitro and in vivo plants. Five arbitrary decamers displayed same banding profile within all the micropropagated plants and in vivo explant donor. The cytological and molecular analysis complemented and compared well and showed no genomic alterations in the plants regenerated through shoot bud differentiation. High multiplication frequency, molecular, cytological and phenotypic stability ensures the efficacy of the protocol developed for the production and conservation of this important endangered medicinal herb. Springer-Verlag, 2005 Chlorophytum arundinaceum nationallicence Medicinal herb nationallicence Endangered nationallicence In vitro regeneration nationallicence Genetic fidelity nationallicence Meiosis nationallicence Mitosis nationallicence RAPD nationallicence Lattoo S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut Bamotra S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut Sapru Dhar R. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut Khan S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut Dhar A. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 499-506 0721-7714 25:6<499 2006 25 299 https://doi.org/10.1007/s00299-005-0103-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0103-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lattoo S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut NATIONALLICENCE 700 1- Bamotra S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut NATIONALLICENCE 700 1- Sapru Dhar R. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut NATIONALLICENCE 700 1- Khan S. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut NATIONALLICENCE 700 1- Dhar A. Genetics and Plant Breeding, Regional Research Laboratory (CSIR), Canal Road, 180 001, Jammu Tawi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 499-506 0721-7714 25:6<499 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer