caa a22 4500 467900035 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0057-6 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0057-6 Isolation of cytoplasts from Satsuma mandarin ( Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast-protoplast fusion [Elektronische Daten] [X. Xu, J. Liu, X. Deng] Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present paper cytoplasts were isolated from cell suspension-derived protoplasts of Satsuma mandarin via ultra-centrifugation in a discontinuous gradient. No nucleus could be detected in the cytoplasts by DAPI (4′, 6-diamidino-2-phenylindole) staining compared with normal protoplasts. The cytoplasts, with high viability and small size, did not divide during solid embedding culture. Cytoplasts of Satsuma mandarin were electrically fused with embryogenic protoplasts of Murcott tangor (C. reticulata × C. sinensis), which led to regeneration of several cell lines. Flow cytometry (FCM) indicated that the cell lines were diploids. Simple sequence repeats (SSR) and cleaved amplified polymorphism sequence (CAPS) showed that the cell lines got their nuclear DNA from the protoplast parent, whereas the cytoplast parent donated the mtDNA, confirming transfer of mtDNA from Satsuma mandarin into Murcott tangor via cytoplast-protoplast fusion though no polymorphism was detected in chloroplast DNA between the fusion partners. This is the first report on isolation and characterization of cytoplasts, together with cytoplast-protoplast fusion in Citrus, which has a potential for citrus cultivar improvement involving cytoplasm transfer via cytoplast-protoplast fusion. Springer-Verlag, 2005 Alloplasmic hybrids nationallicence CAPS nationallicence Citrus unshiu Marc nationallicence Cytoplast nationallicence Cytoplast-protoplast fusion nationallicence FCM nationallicence mtDNA nationallicence SSR nationallicence CAPS : Cleaved amplified polymorphism sequence nationallicence DAPI : 4′, 6-Diamidino-2- phenylindole nationallicence FCM : Flow cytometry nationallicence FDA : Fluorescein diacetate nationallicence MT : Murashige and Tucker nationallicence mtDNA : Mitochondrial DNA nationallicence RFLP : Restriction fragment length polymorphism nationallicence SSR : Simple sequence repeat nationallicence Xu X. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut Liu J. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut Deng X. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 533-539 0721-7714 25:6<533 2006 25 299 https://doi.org/10.1007/s00299-005-0057-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0057-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu X. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut NATIONALLICENCE 700 1- Liu J. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut NATIONALLICENCE 700 1- Deng X. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 533-539 0721-7714 25:6<533 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer