caa a22 4500 467900043 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0065-6 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0065-6 Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors [Elektronische Daten] [Mohammad Ali, Kee-Won Yu, Eun-Joo Hahn, Kee-Yoeup Paek] The effects of methyl jasmonate (MJ) and salicylic acid (SA) on changes of the activities of major antioxidant enzymes, superoxide anion accumulation (O2 −), ascorbate, total glutathione (TG), malondialdehyde (MDA) content and ginsenoside accumulation were investigated in ginseng roots (Panax ginseng L.) in 4 l (working volume) air lift bioreactors. Single treatment of 200 μM MJ and SA to P. ginseng roots enhanced ginsenoside accumulation compared to the control and harvested 3, 5, 7 and 9 days after treatment. MJ and SA treatment induced an oxidative stress in P. ginseng roots, as shown by an increase in lipid peroxidation due to rise in O2 − accumulation. Activity of superoxide dismutase (SOD) was inhibited in MJ-treated roots, while the activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), SOD, guaiacol peroxidase (G-POD), glutathione peroxidase (GPx) and glutathione reductase (GR) were induced in SA-treated roots. A strong decrease in the activity of catalase (CAT) was obtained in both MJ- and SA-treated roots. Activities of ascorbate peroxidase (APX) and glutathione S transferase (GST) were higher in MJ than SA while the contents of reduced ascorbate (ASC), redox state (ASC/(ASC+DHA)) and TG were higher in SA- than MJ-treated roots while oxidized ascorbate (DHA) decreased in both cases. The result of these analyses suggests that roots are better protected against the O2 − stress, thus mitigating MJ and SA stress. The information obtained in this work is useful for efficient large-scale production of ginsenoside by plant-root cultures. Springer-Verlag, 2005 Antioxidant enzymes nationallicence Malondialdehyde content nationallicence Panax ginseng roots nationallicence Saponin nationallicence Superoxide anion nationallicence APX : Ascorbate peroxidase (EC 1.11.1.11) nationallicence CAT : Catalase (EC 1.11.1.6) nationallicence CBN : Chungbuk National University Line 1 nationallicence DHAR : Dehydroascorbate reductase (EC 1.8.5.1) nationallicence DHA : Dehydroascorbate nationallicence GST : Glutathione-S-transferase (EC 2.5.1.18) nationallicence GPx : Glutathione peroxidase (EC 1.11.1.12) nationallicence GR : Glutathione reductase (EC 1.6.4.2) nationallicence G-POD : Guaiacol peroxidase (EC 1.11.1.7) nationallicence MDA : Malondialdehyde nationallicence MDHAR : Monodehydroascorbate reductase (EC 1.6.5.4) nationallicence NBT : Nitroblue tetrazolium nationallicence ROS : Reactive oxygen species nationallicence ASC : Reduced ascorbate nationallicence SOD : Superoxide dismutase (EC 1.15.1.1) nationallicence XTT : Sodium,3,-[1-[phenylamino-carbonyl]-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene-sulfonic acid hydrate nationallicence Ali Mohammad Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut Yu Kee-Won Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 613-620 0721-7714 25:6<613 2006 25 299 https://doi.org/10.1007/s00299-005-0065-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0065-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ali Mohammad Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut NATIONALLICENCE 700 1- Yu Kee-Won Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut NATIONALLICENCE 700 1- Hahn Eun-Joo Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut NATIONALLICENCE 700 1- Paek Kee-Yoeup Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheong-ju, Republic of Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 613-620 0721-7714 25:6<613 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer