caa a22 4500 467900051 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0101-6 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0101-6 Tobacco ribosomal DNA spacer element elevates Bowman-Birk inhibitor expression in tomato plants [Elektronische Daten] [Nir Yakoby, Alison Garvey, Ilya Raskin] Amplification promoting sequence (aps), from tobacco rDNA, was found to induce amplification and enhances the expression of heterologous genes, consequently increasing the expression of transgenic proteins in tobacco. In this report we demonstrate that aps element also affects integration, transcription, and translation of a soybean protease inhibitor, Bowman-Birk inhibitor (BBI), in transgenic tomato plants and quantifies its effects in different expression vectors. A synthetic bbi gene was constructed, based on the wild-type gene containing two independent inhibition sites; trypsin and chymotrypsin. Transformation vectors were designed using two different promoters; the tomato fruit specific E8 promoter and the constitutively active 35S CaMV promoter. These vectors were transformed into ‘Moneymaker' tomato plants. In tomato fruits and leaves, aps caused a 3-fold increase in bbi mRNA levels when compared to the lines without aps. Similar increases were obtained in plants expressing bbi controlled by E8 or 35S CaMV promoters. Also, the level of BBI protein expression in aps-transformed plants was 3 fold-higher than in plants without aps. This is the first report of aps effect on the enhanced gene expression and transgenic protein production in plant other than tobacco. Springer-Verlag, 2005 Agrobacterium tumefaciens nationallicence Amplification promoting sequence nationallicence Bowman-Birk inhibitor nationallicence E8 promoter nationallicence Serine protease inhibitor nationallicence Transgenic tomato plants nationallicence Yakoby Nir Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut Garvey Alison Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut Raskin Ilya Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 573-581 0721-7714 25:6<573 2006 25 299 https://doi.org/10.1007/s00299-005-0101-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0101-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yakoby Nir Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Garvey Alison Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Raskin Ilya Biotech Center, Cook College, Rutgers University, Foran Hall, 59 Dudley Road, 08901-8520, New Brunswick, NJ, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 573-581 0721-7714 25:6<573 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer