caa a22 4500 467900108 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0095-0 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0095-0 Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf [Elektronische Daten] [Myung Kim, Bum-Soo Lee, Jun-Gyo In, Hua Sun, Jae-Ho Yoon, Deok-Chun Yang] The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequences, consisting of 1,167 singletons and 409 contig sequences. BLAST comparisons of the cDNAs in GenBank's non-redundant databases revealed that 2,579 of the 2,896 cDNAs (89.1%) exhibited a high degree of sequence homology to genes from other organisms. The majority of the identified transcripts were found to be genes related with energy, metabolism, subcellular localization, and protein synthesis and transport. The chlorophyll a/b-binding protein ESTs in the ginseng leaf samples manifested a substantially higher level of expression than was observed in other plant leaves. The ESTs involved in ginsenoside biosynthesis were also identified and discussed. Springer-Verlag, 2005 Chlorophyll a/b-binding protein nationallicence Expressed sequence tags nationallicence Ginsenoside nationallicence Leaf nationallicence Panax ginseng nationallicence CAB : chlorophyll a/b-binding protein nationallicence cDNA : complementary DNA nationallicence ESTs : expressed sequence tags nationallicence Kim Myung Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut Lee Bum-Soo Biopia Co., Ltd., 383-4 Singal-ri, Kihung-eup Yongin, 449-598, Kyunggi-do, South Korea aut In Jun-Gyo Biopia Co., Ltd., 383-4 Singal-ri, Kihung-eup Yongin, 449-598, Kyunggi-do, South Korea aut Sun Hua Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut Yoon Jae-Ho Division of Plant Science, Sunchon National University, 540-742, Sunchon, South Korea aut Yang Deok-Chun Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 599-606 0721-7714 25:6<599 2006 25 299 https://doi.org/10.1007/s00299-005-0095-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0095-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Myung Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut NATIONALLICENCE 700 1- Lee Bum-Soo Biopia Co., Ltd., 383-4 Singal-ri, Kihung-eup Yongin, 449-598, Kyunggi-do, South Korea aut NATIONALLICENCE 700 1- In Jun-Gyo Biopia Co., Ltd., 383-4 Singal-ri, Kihung-eup Yongin, 449-598, Kyunggi-do, South Korea aut NATIONALLICENCE 700 1- Sun Hua Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut NATIONALLICENCE 700 1- Yoon Jae-Ho Division of Plant Science, Sunchon National University, 540-742, Sunchon, South Korea aut NATIONALLICENCE 700 1- Yang Deok-Chun Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, 449-701, Kyunggi-do, South Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 599-606 0721-7714 25:6<599 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer