caa a22 4500 467900116 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0109-y doi (NATIONALLICENCE)springer-10.1007/s00299-005-0109-y Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures [Elektronische Daten] [Carolyn Lee-Parsons, Amber Royce] Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100μM) on day 6 and fed loganin (0.30mM), tryptamine (0.15mM), loganin plus tryptamine, or geraniol (0.1-1.0mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition. Springer-Verlag, 2005 Ajmalicine nationallicence Catharanthus roseus nationallicence Loganin nationallicence Methyl jasmonate nationallicence Precursor feeding nationallicence Tryptamine nationallicence Lee-Parsons Carolyn Chemical Engineering Department, 342 Snell Engineering Center, Northeastern University, 360 Huntington Avenue, 02115-5000, Boston, MA, USA aut Royce Amber Chemical Engineering Department, 342 Snell Engineering Center, Northeastern University, 360 Huntington Avenue, 02115-5000, Boston, MA, USA aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 607-612 0721-7714 25:6<607 2006 25 299 https://doi.org/10.1007/s00299-005-0109-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0109-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee-Parsons Carolyn Chemical Engineering Department, 342 Snell Engineering Center, Northeastern University, 360 Huntington Avenue, 02115-5000, Boston, MA, USA aut NATIONALLICENCE 700 1- Royce Amber Chemical Engineering Department, 342 Snell Engineering Center, Northeastern University, 360 Huntington Avenue, 02115-5000, Boston, MA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 607-612 0721-7714 25:6<607 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer