caa a22 4500 467900124 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00299-005-0102-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0102-5 Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi [Elektronische Daten] [S. Oard, F. Enright] Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. β-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of β-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic β-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing β-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression. Springer-Verlag, 2005 Antifungal activity nationallicence Antimicrobial peptides nationallicence Arabidopsis nationallicence β-purothionin nationallicence Plant disease resistance nationallicence Plant promoter nationallicence Oard S. LSU AgCenter Biotechnology Laboratory, Louisiana State University, 115 Wilson Bldg., 70803, Baton Rouge, LA, USA aut Enright F. LSU AgCenter Biotechnology Laboratory, Louisiana State University, 115 Wilson Bldg., 70803, Baton Rouge, LA, USA aut Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 561-572 0721-7714 25:6<561 2006 25 299 https://doi.org/10.1007/s00299-005-0102-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0102-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oard S. LSU AgCenter Biotechnology Laboratory, Louisiana State University, 115 Wilson Bldg., 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Enright F. LSU AgCenter Biotechnology Laboratory, Louisiana State University, 115 Wilson Bldg., 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/6(2006-06-01), 561-572 0721-7714 25:6<561 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer