caa a22 4500 467900159 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0142-5 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0142-5 Comparative analysis of 35S and lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis [Elektronische Daten] [Marco Buenrostro-Nava, Peter Ling, John Finer] Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimensional robotics table and a programmable image acquisition system, which was used to semi-continuously monitor gfp gene expression during development of transgenic soybean [Glycine max (L.) Merrill] somatic embryos. Quantitative analysis of GFP expression showed that, during somatic embryo proliferation and early development, expression of lectin-GFP was not detected. During late embryo development, expression of lectin-GFP gradually increased until the levels were similar to those of 35S-GFP. The use of an automated image collection system and image analysis facilitated the frequent monitoring and quantification of gfp gene expression on a large number of samples over an extended period of time. Springer-Verlag, 2006 Green fluorescent protein nationallicence Image analysis nationallicence Promoter analysis nationallicence Robotics nationallicence Buenrostro-Nava Marco Department of Horticulture and Crop Science, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut Ling Peter Department of Food, Agricultural and Biological Engineering, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut Finer John Department of Horticulture and Crop Science, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 920-926 0721-7714 25:9<920 2006 25 299 https://doi.org/10.1007/s00299-006-0142-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0142-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buenrostro-Nava Marco Department of Horticulture and Crop Science, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Ling Peter Department of Food, Agricultural and Biological Engineering, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Finer John Department of Horticulture and Crop Science, The Ohio State University/OARDC, 44691, Wooster, OH, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 920-926 0721-7714 25:9<920 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer