caa a22 4500 467900167 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0141-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0141-6 Development of transgenic pearl millet ( Pennisetum glaucum (L.) R. Br.) plants resistant to downy mildew [Elektronische Daten] [A. Latha, K. Rao, T. Reddy, V. Reddy] Transgenic pearl millet lines expressing pin gene—exhibiting high resistance to downy mildew pathogen, Sclerospora graminicola—were produced using particle-inflow-gun (PIG) method. Shoot-tip-derived embryogenic calli were co-bombarded with plasmids containing pin and bar genes driven by CaMV 35S promoter. Bombarded calli were cultured on MS medium with phosphinothricin as a selection agent. Primary transformants 1T0, 2T0, and 3T0 showed the presence of both bar and pin coding sequences as evidenced by PCR and Southern blot analysis, respectively. T1 progenies of three primary transformants, when evaluated for downy mildew resistance, segregated into resistant and susceptible phenotypes. T1 plants resistant to downy mildew invariably exhibited tolerance to Basta suggesting co-segregation of pin and bar genes. Further, the downy mildew resistant 1T1 plants were found positive for pin gene in Southern and Northern analyses thereby confirming stable integration, expression, and transmission of pin gene. 1T2 progenies of 1T0 conformed to dihybrid segregation of 15 resistant:1 susceptible plants. Springer-Verlag, 2006 Downy mildew nationallicence Particle-inflow-gun method nationallicence Pennisetum glaucum nationallicence Sclerospora graminicola nationallicence Synthetic pin gene nationallicence Transgenic pearl millet nationallicence Latha A. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut Rao K. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut Reddy T. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut Reddy V. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 927-935 0721-7714 25:9<927 2006 25 299 https://doi.org/10.1007/s00299-006-0141-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0141-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Latha A. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Rao K. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Reddy T. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 700 1- Reddy V. Centre for Plant Molecular Biology, Osmania University, 500007, Hyderabad, Andhra Pradesh, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 927-935 0721-7714 25:9<927 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer