caa a22 4500 467900175 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0156-z doi (NATIONALLICENCE)springer-10.1007/s00299-006-0156-z Mannose selection system used for cucumber transformation [Elektronische Daten] [Zhengquan He, ZhenZhen Duan, Wei Liang, Faju Chen, Wei Yao, Hongwei Liang, Chaoyin Yue, Zongxiu Sun, Fan Chen, Jianwu Dai] The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10g/l mannose and 10g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation. Springer-Verlag, 2006 Agrobacterium -mediated transformation nationallicence Cucumis sativus L nationallicence Phosphomannose-isomerase nationallicence Positive selection nationallicence He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Duan ZhenZhen Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Liang Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Chen Faju Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Yao Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Liang Hongwei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Yue Chaoyin Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Sun Zongxiu National Key Laboratory for Rice Biology, China National Rice Research Institute, 310006, Hangzhou, P.R. China aut Chen Fan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Dai Jianwu Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 953-958 0721-7714 25:9<953 2006 25 299 https://doi.org/10.1007/s00299-006-0156-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0156-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Duan ZhenZhen Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Liang Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Chen Faju Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Yao Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Liang Hongwei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Yue Chaoyin Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Sun Zongxiu National Key Laboratory for Rice Biology, China National Rice Research Institute, 310006, Hangzhou, P.R. China aut NATIONALLICENCE 700 1- Chen Fan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 700 1- Dai Jianwu Biotechnology Research Center, China Three Gorges University, 443002, Yichang, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 953-958 0721-7714 25:9<953 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer