caa a22 4500 467900183 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0150-5 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0150-5 Artificial synthesis of interspecific chimeras between tuber mustard ( Brassica juncea ) and cabbage ( Brassica oleracea ) and cytological analysis [Elektronische Daten] [Li-Ping Chen, Ya-Ming Ge, Xue-Yun Zhu] Interspecific chimeras between tuber mustard and red cabbage were obtained by in vitro graft-culture method. Before grafting, 6-day-old seedlings of tuber mustard and red cabbage were vertically half-cut and treated with different concentrations of 6-BA and NAA for 1min, then, they were symmetrically fit together. As a result, sectorial chimeras were initially produced from the united shoot tips. The maximum frequency of chimeral bud formation reached 6.33% when the vertical sections of tuber mustard and cabbage were treated with 2mg/l 6-BA and 1mg/l NAA. When sectorial chimeras were propagated on MS medium containing 1mg/l 6-BA, periclinal and mericlinal chimeras gradually developed. Chimeral shoots were rooted on half-strength MS medium containing 0.1mg/l NAA. The rooted chimeras were acclimatized and transferred to the field for cytological and morphological analysis. The results showed that stomata density in the chimeras was significantly higher than that of their parents, while chloroplast size, starch grain size and number were intermediate between the two parents. The chimeras were further analyzed by flow cytometry, and the results indicated that they contained both sets of parental chromosomes. Moreover, chimeral plants possessed valuable characters from the two parents. Springer-Verlag, 2006 Brassica juncea nationallicence Brassica oleracea nationallicence Chloroplast nationallicence Chromosome number nationallicence Flow cytometry nationallicence Interspecific chimera nationallicence Stomata nationallicence Chen Li-Ping Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut Ge Ya-Ming Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut Zhu Xue-Yun Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 907-913 0721-7714 25:9<907 2006 25 299 https://doi.org/10.1007/s00299-006-0150-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0150-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Li-Ping Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Ge Ya-Ming Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Zhu Xue-Yun Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 907-913 0721-7714 25:9<907 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer