caa a22 4500 467900191 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0147-0 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0147-0 Anther-specific expression of mutated melon ethylene receptor gene Cm-ERS1 [Elektronische Daten] H70A affected tapetum degeneration and pollen grain production in transgenic tobacco plants / [Keita Takada, Kentaro Ishimaru, Hiroshi Kamada, Hiroshi Ezura] To develop a new system for inducible male sterility without any modification of the floral architecture in tobacco plants, a mutated ethylene receptor gene Cm-ERS1/H70A was fused either to the tobacco Nin88 promoter known to function mainly in the tapetum and microspore or to the CaMV 35S promoter known to be a constitutive promoter. The fusion genes pNin88::Cm-ERS1/H70A and p35S::Cm-ERS1/H70A were introduced in tobacco plants, which generated two independent transformants. Transformants with 35S::Cm-ERS1/H70A produced less normal pollen and had modified floral architecture while those with Nin88::Cm-ERS1/H70A produced less normal pollen without modification of floral architecture. Histological observations of anthers at stage 2 showed that tapetum degeneration in NH70A #8 and H70A #2 transformants occurred later than in wild types, strongly indicating that the expression of the mutated gene was involved in this delay. These results suggest that the tapetum-specific expression of a mutated ethylene receptor gene is a potential strategy for inducing male sterility in transgenic plants. Springer-Verlag, 2006 Ethylene nationallicence Floral architecture nationallicence Heterostyly nationallicence Male sterility nationallicence Nicotiana tabacum nationallicence Tapetum nationallicence Takada Keita Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut Ishimaru Kentaro Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut Kamada Hiroshi Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut Ezura Hiroshi Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 936-941 0721-7714 25:9<936 2006 25 299 https://doi.org/10.1007/s00299-006-0147-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0147-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takada Keita Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Ishimaru Kentaro Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Kamada Hiroshi Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Ezura Hiroshi Gene Research Center, University of Tsukuba, Ten-nodai 1-1-1, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 936-941 0721-7714 25:9<936 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer