caa a22 4500 467900205 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0151-4 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0151-4 Stress- and development-induced expression of spliced and unspliced transcripts from two highly similar dehydrin 1 genes in V. riparia and V. vinifera [Elektronische Daten] [Huogen Xiao, Annette Nassuth] Dehydrins are proteins that accumulate in vegetative tissues subjected to various dehydrating stress conditions such as cold, drought, and salinity and in seeds at later stages of embryogenesis. Here, we report on two highly identical dehydrin genes, DHN1a and DHN1b, in wild and cultivated grapes, Vitis riparia and Vitis vinifera, and their expression in different tissues and under different environmental conditions. The two genes and their transcripts can easily be distinguished by RT-PCR because DHN1b has an 18bp deletion compared to DHN1a. V. riparia expressed only DHN1a; V. vinifera expressed both DHN1a and DHN1b. Spliced transcripts, DHN1-S, encoding a putative YSK2-type dehydrin were present in low amounts in control leaves, but in high amounts in buds and seeds. Unspliced transcripts, DHN1-U, accumulated to high levels in buds and seeds. Cold, drought, and ABA treatment increased accumulation of both DHN1-S and DHN1-U in leaves, whereas short-day treatment increased only DHN1-S. The possible relation of these results with the difference in freezing stress tolerance between V. riparia and V. vinifera is discussed. Springer-Verlag, 2006 Abiotic stress nationallicence Alternative splicing nationallicence Dehydrin nationallicence Vitis riparia nationallicence Vitis vinifera nationallicence Xiao Huogen Department of Molecular and Cellular Biology, University of Guelph, ON N1G 2W1, Guelph, Canada aut Nassuth Annette Department of Molecular and Cellular Biology, University of Guelph, ON N1G 2W1, Guelph, Canada aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 968-977 0721-7714 25:9<968 2006 25 299 https://doi.org/10.1007/s00299-006-0151-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0151-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Huogen Department of Molecular and Cellular Biology, University of Guelph, ON N1G 2W1, Guelph, Canada aut NATIONALLICENCE 700 1- Nassuth Annette Department of Molecular and Cellular Biology, University of Guelph, ON N1G 2W1, Guelph, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 968-977 0721-7714 25:9<968 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer