caa a22 4500 467900221 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-005-0108-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0108-z Plant regeneration through somatic embryogenesis from tissues of mature oak trees: true-to-type conformity of plantlets by RAPD analysis [Elektronische Daten] [S. Valladares, C. Sánchez, M. Martínez, A. Ballester, A. Vieitez] Somatic embryogenesis was induced in expanding leaf explants excised from epicormic shoots forced from branch segments taken at four different times of year from a mature oak (Quercus robur L.). Branch segments 2-4cm in diameter produced most shoots when collected in March. Somatic embryos were induced on explants derived from branches of all collection dates, although collection in November seemed to afford the best results. Germination and conversion ability of embryos of embryogenic lines derived from six oak trees depended heavily on genotype, conversion rates ranging from 0 to 70%. RAPD analyses found no evidence of genetic variation either within or between the embryogenic lines established from three of these trees, or between these lines and the trees of origin, or between somatic embryo derived plantlets and the trees of origin. The embryogenic system used in this study appears to be suitable for true-to-type clonal propagation of mature oak genotypes. Springer-Verlag, 2005 Genetic stability nationallicence Pedunculate oak nationallicence Quercus robur nationallicence RAPD analysis nationallicence Somaclonal variation nationallicence Somatic embryogenesis nationallicence Valladares S. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Sánchez C. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Martínez M. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Ballester A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Vieitez A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 879-886 0721-7714 25:9<879 2006 25 299 https://doi.org/10.1007/s00299-005-0108-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0108-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valladares S. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Sánchez C. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Martínez M. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Ballester A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Vieitez A. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 879-886 0721-7714 25:9<879 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer