caa a22 4500 467900248 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0152-3 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0152-3 Isolation and genetic mapping of a Coffea canephora phenylalanine ammonia-lyase gene ( CcPAL1 ) and its involvement in the accumulation of caffeoyl quinic acids [Elektronische Daten] [Venkataramaiah Mahesh, Jean Rakotomalala, Lénaïg Le Gal, Hélène Vigne, Alexandre de Kochko, Serge Hamon, Michel Noirot, Claudine Campa] Biosynthesis of caffeoylquinic acids occurs via the phenylpropanoid pathway in which the phenylalanine ammonia-lyase (PAL) acts as a key-control enzyme. A full-length cDNA (pF6), corresponding to a PAL gene (CcPAL1), was isolated by screening a Coffea canephora fruit cDNA library and its corresponding genomic sequence was characterized. Amplification of total DNA from seven Coffea species revealed differences in intronic length. This interspecific polymorphism was used to locate the gene on a genetic map established for a backcross progeny between Coffea pseudozanguebariae and C. dewevrei. The CcPAL1 gene was found on the same linkage group, but genetically independent, as a caffeoyl-coenzyme A-O-methyltransferase gene, another gene intervening in the phenylpropanoid pathway. In the same backcross, a lower caffeoylquinic acid content was observed in seeds harvested from plants harbouring the C. pseudozanguebariae CcPAL1 allele. Involvement of the CcPAL1 allelic form in the differential accumulation of caffeoylquinic acids in coffee green beans is then discussed. Springer-Verlag, 2006 Chlorogenic acid nationallicence Coffea nationallicence Genetic diversity nationallicence Gene structure nationallicence Phenylalanine ammonia-lyase nationallicence Mahesh Venkataramaiah IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Rakotomalala Jean IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Le Gal Lénaïg IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Vigne Hélène IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut de Kochko Alexandre IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Hamon Serge IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Noirot Michel CIRAD, Pôle 3P, Ligne Paradis, 97.410, St. Pierre, France aut Campa Claudine IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 986-992 0721-7714 25:9<986 2006 25 299 https://doi.org/10.1007/s00299-006-0152-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0152-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahesh Venkataramaiah IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Rakotomalala Jean IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Le Gal Lénaïg IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Vigne Hélène IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- de Kochko Alexandre IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Hamon Serge IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Noirot Michel CIRAD, Pôle 3P, Ligne Paradis, 97.410, St. Pierre, France aut NATIONALLICENCE 700 1- Campa Claudine IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 986-992 0721-7714 25:9<986 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer