caa a22 4500 467900264 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0162-1 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0162-1 Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture [Elektronische Daten] [F. Leal, J. Loureiro, E. Rodriguez, M. Pais, C. Santos, O. Pinto-Carnide] Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P≤0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17pg/2C (cv. ‘Tinta Barroca' and ‘Viosinho') to 1.26pg/2C (cv. ‘Cabernet Sauvignon'). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content. Springer-Verlag, 2006 Flow cytometry nationallicence Grapevine nationallicence Ploidy variability nationallicence Somaclonal variation nationallicence Somatic embryogenesis nationallicence Vitis nationallicence ACM : Anther culture medium nationallicence BAP : Benzylaminopurine nationallicence CRBC : Chicken red blood cells nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence FCM : Flow cytometry nationallicence FL : Linear-fluorescence light scatter nationallicence MS : Murashige and Skoog nationallicence NOA : β-Naphtoxyacetic acid nationallicence PI : Propidium iodide nationallicence PVP : Polyvinylpyrrolidone nationallicence SE : Somatic embryogenesis nationallicence Leal F. Center of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, Ap. 1013, 5001-801, Vila Real, Portugal aut Loureiro J. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Rodriguez E. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Pais M. Unit of Molecular Biology and Plant Biotechnology (ICAT), Ed. ICAT, Campo Grande, 1749-016, Lisboa, Portugal aut Santos C. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Pinto-Carnide O. Center of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, Ap. 1013, 5001-801, Vila Real, Portugal aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 978-985 0721-7714 25:9<978 2006 25 299 https://doi.org/10.1007/s00299-006-0162-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0162-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leal F. Center of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, Ap. 1013, 5001-801, Vila Real, Portugal aut NATIONALLICENCE 700 1- Loureiro J. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Rodriguez E. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Pais M. Unit of Molecular Biology and Plant Biotechnology (ICAT), Ed. ICAT, Campo Grande, 1749-016, Lisboa, Portugal aut NATIONALLICENCE 700 1- Santos C. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Pinto-Carnide O. Center of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, Ap. 1013, 5001-801, Vila Real, Portugal aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 978-985 0721-7714 25:9<978 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer