caa a22 4500 467900280 CHVBK 20180406152815.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00299-006-0131-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0131-8 Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers [Elektronische Daten] [W. Guo, L. Gong, Z. Ding, Y. Li, F. Li, S. Zhao, B. Liu] Codonopsis lanceolata Benth. et Hook. f., commonly known as bonnet bellflower, is a high-valued herb medicine and vegetable. In this study, a large number of plants were regenerated via organogenesis from immature seed-derived calli in C. lanceolata by a simple and efficient method. Compared with the mother donor plant, the regenerated plants did not exhibit visible phenotypic variations in six major morphological traits examined at the stage of one-season-maturity under field conditions. To gain insight into the genomic stability of these regenerated plants, 63 individuals were randomly tagged among a population of more than 2,000 regenerants, and were compared with the single mother donor plant by two molecular markers, the inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD). Apparent genomic variation was detected in the 63 regenerants, whereas preexisting heterozygosiy in the donor plant was deemed minimal by testing 30 seedlings germinated from selfed seeds of the same donor plant. The percentages of polymorphic bands (PPB) in the ISSR and RAPD analysis were respectively 15.7 and 24.9% for the 63 regenerated plants. Cluster analysis indicates that the genetic similarity values calculated on the basis of RAPD and ISSR data among the 64 plants (63 regenerated and one donor) were respectively 0.894 and 0.933, which allow classification of the plants into distinct groups. Nineteen randomly isolated bands underlying the changed RAPD or ISSR patterns were sequenced, and three of them showed significant homology to known-function genes. Detailed pairwise sequence comparison at one locus between the donor plant and a regenerant revealed that insertion of two short (24 and 19bp) stretches of nucleotides in the regenerated plant relative to the donor plant occurred in an apparently stochastic manner. Springer-Verlag, 2006 Codonopsis lanceolata nationallicence Genomic instability organogenesis nationallicence ISSR nationallicence RAPD nationallicence Sequence change nationallicence Guo W. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Gong L. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Ding Z. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Li Y. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Li F. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Zhao S. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Liu B. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 896-906 0721-7714 25:9<896 2006 25 299 https://doi.org/10.1007/s00299-006-0131-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0131-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo W. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Gong L. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Ding Z. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Li Y. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Li F. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Zhao S. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 700 1- Liu B. Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, 130024, Changchun, China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/9(2006-09-01), 896-906 0721-7714 25:9<896 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer