caa a22 4500 467900302 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-005-0112-3 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0112-3 Alcohol-inducible gene expression in transgenic Populus [Elektronische Daten] [S. Filichkin, R. Meilan, V. Busov, C. Ma, A. Brunner, S. Strauss] We tested the efficiency and optimized the conditions for controlled alcohol-inducible transgene expression in Populus using gus as a reporter gene. Specificity of induction, efficiency in different organs, effect of three chemical inducers, and induction methods were tested using up to 10 independent transgenic events generated in two different Populus genotypes. The optimal inducer concentration and the duration of induction period were determined in dose-response and in time-course experiments. Under in vitro conditions, β-glucuronidase (GUS) induction was efficient both in the aerial parts and in the roots of regenerated plantlets. Among the chemical inducers tested, ethanol was the most effective activator with no apparent phytotoxicity when concentrations were at or below 2%. After 5 days of treatment, fluorometrically-determined the GUS activity could be detected when inducing with ethanol at concentrations as low as 0.5%. Prolonged induction by ethanol vapors significantly increased the GUS activity in leaves from both the tissue culture plants and greenhouse-grown plants. Springer-Verlag, 2005 Poplar nationallicence Aspen nationallicence Cottonwood nationallicence alc system nationallicence Functional genomics nationallicence Filichkin S. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut Meilan R. Department of Forestry and Natural Resources, Purdue University, 47907, West Lafayette, IN, USA aut Busov V. School of Forest Resources and Environmental Science, Michigan Technological University, 49931, Houghton, MI, USA aut Ma C. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut Brunner A. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut Strauss S. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 660-667 0721-7714 25:7<660 2006 25 299 https://doi.org/10.1007/s00299-005-0112-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0112-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Filichkin S. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut NATIONALLICENCE 700 1- Meilan R. Department of Forestry and Natural Resources, Purdue University, 47907, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- Busov V. School of Forest Resources and Environmental Science, Michigan Technological University, 49931, Houghton, MI, USA aut NATIONALLICENCE 700 1- Ma C. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut NATIONALLICENCE 700 1- Brunner A. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut NATIONALLICENCE 700 1- Strauss S. Department of Forest Science, Oregon State University, 97331, Corvallis, OR, US aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 660-667 0721-7714 25:7<660 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer