caa a22 4500 467900345 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-005-0111-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0111-4 Occurrence of tetraploidy in Nicotiana attenuata plants after Agrobacterium -mediated transformation is genotype specific but independent of polysomaty of explant tissue [Elektronische Daten] [Ben Bubner, Klaus Gase, Beatrice Berger, Dirk Link, Ian Baldwin] Genotypes of Nicotiana attenuata collected from Utah and Arizona were transformed with 17 different vectors (14 unpublished vectors based on 3 new backbone vectors) using an Agrobacterium-mediated procedure to functionally analyze genes important for plant-insect interactions. None of the 51 T1-T3 transgenic Utah lines analyzed by the flow cytometry were tetraploid, as opposed to 18 of 33 transgenic Arizona lines (55%). Analysis of T0 regenerants transformed with the same vector carrying an inverted repeat (IR) N. attenuata pro-systemin construct confirmed the genotype dependency of tetraploidization: none of the 23 transgenic Utah lines were tetraploid but 31 (72%) of 43 transgenic Arizonas were tetraploid. We tested the hypothesis that the differences in polysomaty of the explant tissues accounted for genotype dependency of tetraploid formation by measuring polysomaty levels in different seedling tissues. Hypocotyls, cotyledons, and roots of Utah and Arizona genotypes contained similar percentages of 4C nuclei (61 and 60; 7 and 5; and 58 and 61%, respectively). Since we used hypocotyls as explant sources and the nonoccurrence of tetraploid Utah transformants does not correspond to the high percentage of 4C nuclei in Utah hypocotyls, we can rule out a direct relationship between tetraploid formation and polysomaty level. We hypothesize that the difference between the Utah and Arizona genotypes results from the failure of polyploid Utah callus to regenerate into fully competent plants. We propose that future work on post-transformation polyploidy concentrate on the processes that occur during callus formation and plant regeneration from callus. Springer-Verlag, 2005 Flow cytometry nationallicence Polyploidy nationallicence Polysomaty nationallicence Transformation nationallicence Transgenic plants nationallicence Bubner Ben Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut Gase Klaus Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut Berger Beatrice Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut Link Dirk Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut Baldwin Ian Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 668-675 0721-7714 25:7<668 2006 25 299 https://doi.org/10.1007/s00299-005-0111-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0111-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bubner Ben Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut NATIONALLICENCE 700 1- Gase Klaus Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut NATIONALLICENCE 700 1- Berger Beatrice Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut NATIONALLICENCE 700 1- Link Dirk Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut NATIONALLICENCE 700 1- Baldwin Ian Max-Planck-Institut für Chemische Ökologie, Hans-Knöll-Straße 8, 07745, Jena, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 668-675 0721-7714 25:7<668 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer