caa a22 4500 467900353 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-005-0072-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0072-7 Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis [Elektronische Daten] [Rongrong Liu, Shaohua Xu, Jialin Li, Yuanlei Hu, Zhongping Lin] An important traditional Chinese medicine herb, Astragalus membranaceus var. Mongholicus, whose dried root is known as Radix astragali ("Huangqi” in Chinese), has high flavonoid content as an essential active constituent. Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the first and also a rate-limiting step in phenylpropanoid pathway, which supplies precursors for a variety of secondary metabolites including flavonoids. A PAL gene, designated AmPAL1 (GenBank accession no. AY986506), was isolated from A. membranaceus var. Mongholicus with a full-length cDNA of 2562 nucleotides and an open reading frame of 2154bp. Northern blot analysis revealed that AmPAL1 expressed universally in different organs, and its expression was markedly induced by UV irradiation, mechanical wounding, and white light irradiation on etiolated seedlings, with some distinctive responsive properties. Content of a typical flavonoid, quercetin, in A. membranaceus var. Mongholicus of different ages correlated with PAL enzymatic activity. Transgenic tobacco plants harboring AmPAL1 under the control of the CaMV35S promoter showed significantly increased PAL activity and correlatively increased quercetin content than those in non-transformed plants. These results indicate that PAL is maybe a key point for flux into flavonoid biosynthesis in the genetic control of secondary metabolism in A. membranaceus var. Mongholicus. Springer-Verlag, 2005 Phenylalanine ammonia-lyase nationallicence Astragalus membranaceus var. Mongholicus nationallicence Expression profile nationallicence Flavonoid biosynthesis nationallicence Liu Rongrong National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut Xu Shaohua National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut Li Jialin National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut Hu Yuanlei National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut Lin Zhongping National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 705-710 0721-7714 25:7<705 2006 25 299 https://doi.org/10.1007/s00299-005-0072-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0072-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Rongrong National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xu Shaohua National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Jialin National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Hu Yuanlei National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Lin Zhongping National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 705-710 0721-7714 25:7<705 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer