caa a22 4500 467900361 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-006-0119-4 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0119-4 Molecular cloning, expression and characterization of a glycosyltransferase from rice [Elektronische Daten] [Jae Ko, Bong Kim, Hor-Gil Hur, Yoongho Lim, Joong-Hoon Ahn] Secondary plant metabolites undergo several modification reactions, including glycosylation. Glycosylation, which is mediated by UDP-glycosyltransferase (UGT), plays a role in the storage of secondary metabolites and in defending plants against stress. In this study, we cloned one of the glycosyltransferases from rice, RUGT-5 resulting in 40-42% sequence homology with UGTs from other plants. RUGT-5 was functionally expressed as a glutathione S-transferase fusion protein in Escherichia coli and was then purified. Eight different flavonoids were used as tentative substrates. HPLC profiling of reaction products displayed at least two peaks. Glycosylation positions were located at the hydroxyl groups at C-3, C-7 or C-4′ flavonoid positions. The most efficient substrate was kaempferol, followed by apigenin, genistein and luteolin, in that order. According to in vitro results and the composition of rice flavonoids the in vivo substrate of RUGT-5 was predicted to be kaempferol or apigenin. To our knowledge, this is the first time that the function of a rice UGT has been characterized. Springer-Verlag, 2006 Flavonoids nationallicence Glycoslytransferase nationallicence Oryza sativa nationallicence IPTG: : Isopropyl-β-d-thiogalactopyran oside nationallicence PCR: : Polymerase chain reaction nationallicence UGT: : Uridine diphosphate dependent glycosyltransferase nationallicence Ko Jae Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Kim Bong Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Hur Hor-Gil Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut Lim Yoongho Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Ahn Joong-Hoon Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 741-746 0721-7714 25:7<741 2006 25 299 https://doi.org/10.1007/s00299-006-0119-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0119-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ko Jae Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Bong Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Hur Hor-Gil Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut NATIONALLICENCE 700 1- Lim Yoongho Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Ahn Joong-Hoon Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 741-746 0721-7714 25:7<741 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer