caa a22 4500 46790037X CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-006-0126-5 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0126-5 Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavistin [Elektronische Daten] [Vaibhav Tiwari, Kavindra Tiwari, Brahma Deo Singh] A mass in vitro propagation system devoid of growth regulators for Bacopa monniera (L.) Wettst., a traditional Indian medicinal plant, has been developed. Direct shoot bud regeneration was induced by culturing internode and leaf explants on Murashige and Skoog's (MS) medium supplemented with an antibiotic (trimethoprim) or a fungicide (bavistin). Bavistin showed a marked cytokinin-like activity, as evident from high number of shoot buds induced in node, internode and leaf explants. Optimum adventitious shoot buds induction occurred at 300mg/l bavistin from internode explants. In vitro regenerated shoots were elongated and rooted before transferred to field with 85% survival. The regeneration protocol developed in this study illustrates the usefulness of additives for mass propagation and germplasm conservation of B. monniera. Springer-Verlag, 2006 Antibiotic nationallicence Bacopa monniera nationallicence Brahmi nationallicence Fungicide nationallicence Medicinal plant nationallicence Shoot regeneration nationallicence Tiwari Vaibhav Faculty of Sciences, School of Biotechnology, Banaras Hindu University, 221005, Varanasi, UP, India aut Tiwari Kavindra Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, 221005, Varanasi, UP, India aut Deo Singh Brahma Faculty of Sciences, School of Biotechnology, Banaras Hindu University, 221005, Varanasi, UP, India aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 629-635 0721-7714 25:7<629 2006 25 299 https://doi.org/10.1007/s00299-006-0126-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0126-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tiwari Vaibhav Faculty of Sciences, School of Biotechnology, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 700 1- Tiwari Kavindra Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 700 1- Deo Singh Brahma Faculty of Sciences, School of Biotechnology, Banaras Hindu University, 221005, Varanasi, UP, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 629-635 0721-7714 25:7<629 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer