caa a22 4500 467900388 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-005-0110-5 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0110-5 Characterisation of basal resistance (BR) by expression patterns of newly isolated representative genes in tobacco [Elektronische Daten] [Agnes Szatmari, Péter Ott, Gabriella Varga, Eszter Besenyei, Arnold Czelleng, Zoltán Klement, Zoltán Bozsó] Increasing evidence indicates that plants, like animals, use basal resistance (BR), a component of the innate immune system, to defend themselves against foreign organisms. Contrary to the hypersensitive reaction (HR)-type cell death, recognition in the case of BR is unspecific, as intruders are recognised based on their common molecular patterns. Induction of BR is not associated with visible symptoms, in contrast to the HR-type cell death. To analyse the early events of BR in tobacco plants we have carried out a subtractive hybridisation between leaves treated with the HR-negative mutant strain Pseudomonas syringae pv. syringae 61 hrcC and non-treated control leaves. Random sequencing from the 304 EBR clones yielded 20 unique EST-s. Real-time PCR has proved that 8 out of 10 clones are activated during BR. Six of these EST-s were further analyzed. Gene expression patterns in a time course showed early peaks of most selected genes at 3-12h after inoculation (hpi), which coincided with the development-time of BR. Upon treatments with different types of bacteria we found that incompatible pathogens, their hrp mutants, as well as non-pathogens induce high levels of expression while virulent pathogens induce only a limited gene-expression. Plant signal molecules like salicylic acid, methyl jasmonate, ethylene and spermine, known to be involved in plant defense were not able to induce the investigated genes, therefore, an unknown signalling mechanism is expected to operate in BR. In summary, we have identified representative genes associated with BR and have established important features of BR by analysing gene-expression patterns. Springer-Verlag, 2005 Basal resistance nationallicence Pseudomonas nationallicence Tobacco nationallicence Innate immunity nationallicence Hypersensitive reaction nationallicence Szatmari Agnes Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Ott Péter Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Varga Gabriella Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Besenyei Eszter Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Czelleng Arnold Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Klement Zoltán Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Bozsó Zoltán Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 728-740 0721-7714 25:7<728 2006 25 299 https://doi.org/10.1007/s00299-005-0110-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0110-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szatmari Agnes Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Ott Péter Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Varga Gabriella Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Besenyei Eszter Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Czelleng Arnold Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Klement Zoltán Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 700 1- Bozsó Zoltán Plant Protection Institute, Hungarian Academy of Sciences, Herman O. u. 15., 1022, Budapest, Hungary aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 728-740 0721-7714 25:7<728 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer