caa a22 4500 467900418 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-006-0134-5 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0134-5 Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion [Elektronische Daten] [Ksenija Taski-Ajdukovic, Dragana Vasic, Nevena Nagl] Helianthus maximiliani is one of the wild Helianthus species with the genes for resistance to many pathogens including Sclerotinia sclerotiorum. Unfortunately, a transfer of disease resistance genes from this species into the cultivated sunflower is limited by its poor crossability with the cultivated sunflower and sterility of interspecific hybrids. To overcome this problem, mesophyll protoplasts of Sclerotinia sclerotiorum-resistant clone of H. maximiliani were electrically fused with etiolated hypocotyl protoplasts of the cultivated sunflower inbred line PH-BC1-91A. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. Developed microcalluses were released from the agarose and transferred into solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2mgl−1 BAP and 0.01mgl−1 NAA after the treatment with a high concentration of 2,4 D for a limited period of time. A morphological and RAPD analysis confirmed a hybrid nature of the regenerated plants. Springer-Verlag, 2006 Electrofusion nationallicence Helianthus nationallicence RAPD analysis nationallicence Regeneration nationallicence Somatic hybridization nationallicence Taski-Ajdukovic Ksenija National Laboratory for Seed Testing, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut Vasic Dragana Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut Nagl Nevena Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 698-704 0721-7714 25:7<698 2006 25 299 https://doi.org/10.1007/s00299-006-0134-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0134-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taski-Ajdukovic Ksenija National Laboratory for Seed Testing, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut NATIONALLICENCE 700 1- Vasic Dragana Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut NATIONALLICENCE 700 1- Nagl Nevena Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, Yugoslavia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 698-704 0721-7714 25:7<698 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer