caa a22 4500 467900426 CHVBK 20180406152816.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00299-006-0133-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0133-6 Effects of codon modification on human BMP2 gene expression in tobacco plants [Elektronische Daten] [Guangli Suo, Bing Chen, Jingyu Zhang, Ziyuan Duan, Zhengquan He, Wei Yao, Chaoyin Yue, Jianwu Dai] Bone morphogenetic protein 2 (BMP2) has great potential in therapeutic applications. We are working on generating transgenic plants as a bioreactor to produce BMP2. We have studied the effects of codon optimization on the expression of human BMP2 (hBMP2) in tobacco plants. Three modified hBMP2 genes were transformed into tobacco under the control of either cauliflower mosaic virus 35S (CaMV35S) promoter or double-CaMV35S promoter plus alfalfa mosaic virus (AMV) enhancer. The fused β-glucuronidase (GUS) reporter gene was used to facilitate the assay of protein expression. The results indicated that codon optimization could increase the protein expression level obviously under CaMV35S promoter. However, under relatively stronger initiation condition (double-CaMV35S promoter plus AMV enhancer), only the gene with the lowest degree of codon optimization could increase the protein expression level. Our findings suggest that the action of codon optimization may be influenced by the factors of promoter strength and A+T content in tobacco plants. Springer-Verlag, 2006 Codon optimization nationallicence Codon usage nationallicence Human BMP2 nationallicence Transgenic tobacco nationallicence Suo Guangli Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology and the Graduate School, Chinese Academy of Sciences, 100080, Beijing, People's Republic of China aut Chen Bing Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut Zhang Jingyu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut Duan Ziyuan Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut Yao Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut Yue Chaoyin Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut Dai Jianwu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 689-697 0721-7714 25:7<689 2006 25 299 https://doi.org/10.1007/s00299-006-0133-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0133-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suo Guangli Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology and the Graduate School, Chinese Academy of Sciences, 100080, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Bing Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jingyu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Duan Ziyuan Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- He Zhengquan Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut NATIONALLICENCE 700 1- Yao Wei Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut NATIONALLICENCE 700 1- Yue Chaoyin Biotechnology Research Center, China Three Gorges University, 443002, Yichang, People's Republic of China aut NATIONALLICENCE 700 1- Dai Jianwu Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 100080, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/7(2006-07-01), 689-697 0721-7714 25:7<689 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer